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泰国食蟹猕猴()中的分子检测

Molecular Detection of among Long-Tailed Macaques () in Thailand.

作者信息

Sricharern Wanat, Kaewchot Supakarn, Saengsawang Phirabhat, Kaewmongkol Sarawan, Inpankaew Tawin

机构信息

Center for Agricultural Biotechnology, Kamphaengsaen Campus, Kasetsart University, Nakhonpathom 73140, Thailand.

Center of Excellence on Agricultural Biotechnology, Science and Technology Postgraduate Education and Research Development Office, Commission on Higher Education, Ministry of Education, Science, Research Innovation (AG-BIO/PERDO-CHE), Bangkok 10900, Thailand.

出版信息

Pathogens. 2021 May 19;10(5):629. doi: 10.3390/pathogens10050629.

Abstract

is a zoonotic pathogen with a worldwide distribution. Humans and non-human primates are considered to be natural reservoir hosts for However, information on the molecular epidemiology of this organism is very limited in regard to long-tailed macaques () in Thailand. Therefore, this study aimed to investigate the occurrence and genetic diversity of spp. among long-tailed macaques in Thailand. In total, 856 blood samples were collected from long-tailed macaques in Thailand. All specimens were screened for spp. using a polymerase chain reaction (PCR) assay targeting the 16S rRNA, and genes. All positive samples were further analyzed based on nucleotide sequencing, phylogenetic analysis and multiple sequence alignment analysis. Only one macaque showed a positive result in the PCR assays based on the 16S rRNA, and genes. Nucleotide sequencing and phylogenetic analysis revealed that the obtained sequences were closely related to previously detected in non-human primates. Single-nucleotide polymorphisms (SNPs) were detected in the and gene sequences. This study revealed that long-tailed macaques in Thailand carried Despite the low infection rate detected, long-tailed macaques may be a reservoir of .

摘要

是一种分布于全球的人畜共患病原体。人类和非人灵长类动物被认为是其自然宿主。然而,关于泰国长尾猕猴中这种生物体的分子流行病学信息非常有限。因此,本研究旨在调查泰国长尾猕猴中 spp. 的发生情况和遗传多样性。总共从泰国的长尾猕猴中采集了856份血液样本。使用针对16S rRNA、 和 基因的聚合酶链反应(PCR)检测法对所有标本进行spp. 筛查。所有阳性样本进一步基于核苷酸测序、系统发育分析和多序列比对分析进行分析。在基于16S rRNA、 和 基因的PCR检测中,只有一只猕猴呈阳性结果。核苷酸测序和系统发育分析表明,获得的序列与先前在非人灵长类动物中检测到的 密切相关。在 和 基因序列中检测到单核苷酸多态性(SNP)。本研究表明泰国的长尾猕猴携带 。尽管检测到的感染率较低,但长尾猕猴可能是 的宿主。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fa8/8160695/867fcee4e3c6/pathogens-10-00629-g001.jpg

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