Rodríguez Jose M, Diez M Jose, Sierra Matilde, Garcia Juan J, Fernandez Nelida, Diez Raquel, Sahagun Ana M
Pharmacology, Department of Biomedical Sciences, Institute of Biomedicine (IBIOMED), Veterinary Faculty, University of Leon, 24071 Leon, Spain.
Animals (Basel). 2021 May 23;11(6):1514. doi: 10.3390/ani11061514.
Flumequine concentrations in plasma, colon tissue and intestinal contents were evaluated in 12 healthy pigs after oral administration (12 mg/kg every 24 h for 5 consecutive days in drinking water). Plasma, colon tissue and intestinal content samples were collected from animals sacrificed on days 3, 6 and 7. Concentrations were measured by high performance liquid chromatography after having validated the method, following the European Medicines Agency (EMA) requirements. The drug was not detected in any plasma sample. In colon tissue, concentrations were higher on day 3 (0.230 ± 0.033 µg/g, descending colon; 0.156 ± 0.093 µg/g, ascending colon) than on day 6 (0.187 ± 0.123 µg/g, descending colon; 0.107 ± 0.007 µg/g, ascending colon). Concentrations were considerably higher in intestinal contents, again on day 3 (1.349 ± 1.401 µg/g, descending colon; 0.591 ± 0.209 µg/g, ascending colon) than on days 6 (0.979 ± 0.346 µg/g, descending colon; 0.595 ± 0.075 µg/g, ascending colon) and 7 (0.247 ± 0.172 µg/g, descending colon; 0.172 ± 0.086 µg/g, ascending colon). Measured concentrations were lower than those effective against the most common intestinal pathogenic microorganisms in swine and, more specifically, .
在12头健康猪口服给药后（连续5天，每天在饮水中给药12 mg/kg，每24小时一次），评估了血浆、结肠组织和肠内容物中的氟甲喹浓度。在第3、6和7天处死动物后收集血浆、结肠组织和肠内容物样本。按照欧洲药品管理局（EMA）的要求，在验证方法后，通过高效液相色谱法测量浓度。在任何血浆样本中均未检测到该药物。在结肠组织中，第3天的浓度（降结肠为0.230±0.033 µg/g；升结肠为0.156±0.093 µg/g）高于第6天（降结肠为0.187±0.123 µg/g；升结肠为0.107±0.007 µg/g）。肠内容物中的浓度同样在第3天（降结肠为1.349±1.401 µg/g；升结肠为0.591±0.209 µg/g）显著高于第6天（降结肠为0.979±0.346 µg/g；升结肠为0.595±0.075 µg/g）和第7天（降结肠为0.247±0.172 µg/g；升结肠为0.172±0.086 µg/g）。测得的浓度低于对猪中最常见的肠道致病微生物有效的浓度，更具体地说， 。
