Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Nat Prod Res. 2024 Jun;38(12):2155-2159. doi: 10.1080/14786419.2023.2241150. Epub 2023 Jul 30.
GC/MS analysis of (L.) Spreng. and (L.) Jacq. leaves revealed the identification of 73 components, with an evident greater contribution of monoterpenes hydrocarbons to their total volatiles. α-Pinene (37.5%) and β-caryophyllene (27.4%) were the most abundant compounds in leaves and β-phellandrene (40.7%) in leaves, using headspace. β-Phellandrene (33.7%) was the major constituent by leaves where germacrene D (23.8%), and δ-elemene (22.0%) were predominant in leaves, using steam distillation. leaves oil showed quite remarkable cholinesterase inhibitory activity, where oil of leaves showed strong inhibitory activity against AChE (IC=13.2 ± 0.9 µg/mL) and BChE (IC=5.1 ± 0.3 µg/mL). Germacrene D, α-zingiberene, and δ-elemene showed higher affinity to BChE than AChE as revealed from docking scores (S = -5.65 to -6.03 Kcal/mol) for BChE and (S = -5.56 to -6.25 Kcal/mol) for AChE.
GC/MS 分析表明，（L.）Spreng. 和（L.）Jacq. 的叶子中鉴定出 73 种成分，其挥发性成分中以单萜烃的明显贡献更大。α-蒎烯（37.5%）和 β-石竹烯（27.4%）是叶子中含量最丰富的化合物，β-水芹烯（40.7%）是叶子中含量最丰富的化合物。使用顶空法。β-水芹烯（33.7%）是叶子中含量最丰富的化合物，而大根香叶烯 D（23.8%）和 δ-榄香烯（22.0%）则是叶子中含量最丰富的化合物，使用水蒸气蒸馏法。叶子油显示出相当显著的乙酰胆碱酯酶抑制活性，其中叶子油对 AChE（IC=13.2±0.9μg/mL）和 BChE（IC=5.1±0.3μg/mL）具有强烈的抑制活性。大根香叶烯 D、α-姜黄烯和 δ-榄香烯对 BChE 的亲和力高于 AChE，从对接评分（S=-5.65 至-6.03 Kcal/mol）来看，对 BChE 的亲和力高于 AChE，对 AChE 的亲和力为-5.56 至-6.25 Kcal/mol。
