Ramírez-Santos Edwin, Rendon Pedro, Gouvi Georgia, Zacharopoulou Antigone, Bourtzis Kostas, Cáceres Carlos, Bloem Kenneth
Laboratorio El Pino, Programa MOSCAMED, Km 47.5 Carretera a El Salvador, Parque Nacional Laguna El Pino, 06002 Santa Rosa, Guatemala.
International Atomic Energy Agency-Technical Cooperation TCLAC, Programa Moscamed/USDA, Guatemala.
Insects. 2021 May 27;12(6):499. doi: 10.3390/insects12060499.
(Loew) is one of the most destructive insect pests damaging several fruits of economic importance. The sterile insect technique (SIT) is used under an area-wide integrated pest management approach, to suppress these pest populations. Mass rearing facilities were initially established to produce sterile males of bi-sexual strains in support of SIT. The first genetic sexing strain (GSS) for , Tapachula-7, based on pupal color dimorphism, was a key development since the release of males-only significantly increases the SIT efficiency. In this study, we document the development of a novel pupal color-based GSS. Twelve radiation-induced translocation lines were assessed as potential GSS in terms of recombination rates and rearing efficiency at a small scale. The best one, GUA10, was cytogenetically characterized: it was shown to carry a single translocation between the Y chromosome and chromosome 2, which is known to carry the marker. This GSS was further evaluated at medium and large scales regarding its genetic stability, productivity and quality versus Tapachula-7. GUA10 presented better genetic stability, fecundity, fertility, production efficiency, flying ability, and male mating, clear indicators that GUA10 GSS can significantly improve the efficacy and cost-effectiveness of SIT applications against this pest species.
洛氏果蝇是最具破坏性的害虫之一，会损害多种具有经济重要性的果实。在区域综合虫害管理方法中，采用不育昆虫技术（SIT）来抑制这些害虫种群。最初建立大规模饲养设施是为了生产两性品系的不育雄蝇，以支持不育昆虫技术。基于蛹色二态性的首个用于洛氏果蝇的遗传性别品系（GSS）——塔帕丘拉-7，是一项关键进展，因为只释放雄蝇可显著提高不育昆虫技术的效率。在本研究中，我们记录了一种基于蛹色的新型遗传性别品系的培育过程。对12个辐射诱导易位系进行了小规模评估，以确定其作为潜在遗传性别品系的重组率和饲养效率。最佳品系GUA10进行了细胞遗传学特征分析：结果表明它在Y染色体和2号染色体之间存在单一易位，已知2号染色体携带标记。与塔帕丘拉-7相比，对该遗传性别品系在中规模和大规模上进一步评估了其遗传稳定性、生产力和质量。GUA10表现出更好的遗传稳定性、繁殖力、生育力、生产效率、飞行能力和雄蝇交配能力，这些明确指标表明GUA10遗传性别品系可显著提高针对该害虫物种的不育昆虫技术应用的效果和成本效益。
