Centro de Biomateriales, Universidad de La Habana, La Habana 10400, Cuba.
TNI Group, Department of Radiology, Leiden University Medical Center (LUMC), 2333 ZA Leiden, The Netherlands.
Int J Mol Sci. 2021 May 27;22(11):5730. doi: 10.3390/ijms22115730.
Hydrogels obtained from combining different polymers are an interesting strategy for developing controlled release system platforms and tissue engineering scaffolds. In this study, the applicability of sodium alginate-g-(QCL-co-HEMA) hydrogels for these biomedical applications was evaluated. Hydrogels were synthesized by free-radical polymerization using a different concentration of the components. The hydrogels were characterized by Fourier transform-infrared spectroscopy, scanning electron microscopy, and a swelling degree. Betamethasone release as well as the in vitro cytocompatibility with chondrocytes and fibroblast cells were also evaluated. Scanning electron microscopy confirmed the porous surface morphology of the hydrogels in all cases. The swelling percent was determined at a different pH and was observed to be pH-sensitive. The controlled release behavior of betamethasone from the matrices was investigated in PBS media (pH = 7.4) and the drug was released in a controlled manner for up to 8 h. Human chondrocytes and fibroblasts were cultured on the hydrogels. The MTS assay showed that almost all hydrogels are cytocompatibles and an increase of proliferation in both cell types after one week of incubation was observed by the Live/Dead assay. These results demonstrate that these hydrogels are attractive materials for pharmaceutical and biomedical applications due to their characteristics, their release kinetics, and biocompatibility.
由不同聚合物组合而成的水凝胶是开发控制释放系统平台和组织工程支架的一种有趣策略。在这项研究中，评估了海藻酸钠-g-（QCL-co-HEMA）水凝胶在这些生物医学应用中的适用性。水凝胶通过自由基聚合，使用不同浓度的成分合成。通过傅里叶变换红外光谱、扫描电子显微镜和溶胀度对水凝胶进行了表征。还评估了倍他米松的释放以及与软骨细胞和成纤维细胞的体外细胞相容性。扫描电子显微镜证实了所有情况下水凝胶的多孔表面形态。在不同 pH 值下测定了溶胀百分比，并观察到其具有 pH 敏感性。在 PBS 介质（pH = 7.4）中研究了倍他米松从基质中的控制释放行为，药物在长达 8 小时内以受控方式释放。将人软骨细胞和成纤维细胞培养在水凝胶上。MTS 测定表明，几乎所有水凝胶都是细胞相容的，并且通过活/死测定观察到在孵育一周后两种细胞类型的增殖增加。这些结果表明，由于这些水凝胶的特性、释放动力学和生物相容性，它们是用于药物和生物医学应用的有吸引力的材料。
