a Institute of Biomedical and Pharmaceutical Technology, Fuzhou University , Fuzhou , China.
b Bote Biotech. Col., Ltd. , Fuzhou , China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):1710-1721. doi: 10.1080/21691401.2019.1607866.
A dual-layer biomimetic cartilage scaffold was prepared by mimicking the structural design, chemical cues and mechanical characteristics of mature articular cartilage. The surface layer was made from collagen (COL), chitosan (CS) and hyaluronic acid sodium (HAS). The transitional layer with microtubule array structure was prepared with COL, CS and silk fibroin (SF). The PLAG microspheres containing kartogenin (KGN) and the polylysine-heparin sodium nanoparticles containing TGF-β1 (TPHNs) were constructed for the surface, transitional layer, respectively. The SEM result showed that the dual-layer composite scaffold had a double structure similar to natural cartilage. The vitro biocompatibility experiment showed that the biomimetic cartilage scaffold with orientated porous structure was more conducive to the proliferation and adhesion of BMSCs. A rabbit KOA cartilage defect model was established and biomimetic cartilage scaffolds were implanted in the defect area. Compared with the surface layer and transitional layer scaffolds group, the results of dual-layer biomimetic cartilage scaffold group showed that the defects had been completely filled, the boundary between new cartilage and surrounding tissue was difficult to identify, and the morphology of cells in repair tissue was almost in accordance with the normal cartilage after 16 weeks. All those results indicated that the biomimetic cartilage scaffold could effectively repair the defect of KOA, which is related to the fact that the scaffold could guide the morphology, orientation, and proliferation and differentiation of BMSCs. This work could potentially lead to the development of multilayer scaffolds mimicking the zonal organization of articular cartilage.
双层仿生软骨支架通过模拟成熟关节软骨的结构设计、化学线索和机械特性来制备。表面层由胶原蛋白（COL）、壳聚糖（CS）和透明质酸钠（HAS）组成。具有微管阵列结构的过渡层由 COL、CS 和丝素蛋白（SF）制备。PLAG 微球包载卡托利筋素（KGN）和聚赖氨酸-肝素钠纳米粒（TPHNs）分别构建用于表面层和过渡层。SEM 结果表明，双层复合支架具有类似于天然软骨的双层结构。体外生物相容性实验表明，具有定向多孔结构的仿生软骨支架更有利于 BMSCs 的增殖和黏附。建立了兔 KOA 软骨缺损模型，并将仿生软骨支架植入缺损区域。与表面层和过渡层支架组相比，双层仿生软骨支架组的结果表明，缺损已完全填充，新软骨与周围组织之间的边界难以识别，修复组织中细胞的形态与正常软骨几乎一致，16 周后。所有这些结果表明，仿生软骨支架能够有效修复 KOA 缺陷，这与支架能够引导 BMSCs 的形态、取向、增殖和分化有关。这项工作可能会导致模仿关节软骨分区组织的多层支架的发展。
