Feng Tianshu, Hilal Mian Gul, Wang Yijie, Zhou Rui, Yu Qiaoling, Qu Jiapeng, Li Huan
School of Public Health, Lanzhou University, Lanzhou 730000, China.
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China.
Microorganisms. 2021 May 27;9(6):1152. doi: 10.3390/microorganisms9061152.
Gut microbiomes play important functional roles in human health and are also affected by many factors. However, few studies concentrate on gut microbiomes under exercise intervention. Additionally, antibiotic resistance genes (ARGs) carried by gut microbiomes may constantly pose a threat to human health. Here, ARGs and microbiomes of Chinese and Pakistanis participants were investigated using 16S rRNA gene sequencing and high-throughput quantitative PCR techniques. The exercise had no impact on gut microbiomes in the 12 individuals investigated during the observation period, while the different distribution of gut microbiomes was found in distinct nationalities. Overall, the dominant microbial phyla in the participants' gut were Bacteroidota, Firmicutes and Proteobacteria. Some genera such as and were more abundant in Pakistani participants and some other genera such as and were more abundant in Chinese participants. The microbial diversity in Chinese was higher than that in Pakistanis. Furthermore, microbial community structures were also different between Chinese and Pakistanis. For ARGs, the distribution of all detected ARGs is not distinct at each time point. Among these ARGs, was distributed differently in Chinese and Pakistani participants, and some ARGs such as and are positively correlated with several dominant microbiomes, particularly Bacteroidota and Firmicutes bacteria that did not fluctuate over time.
肠道微生物群在人类健康中发挥着重要的功能作用，同时也受到多种因素的影响。然而，很少有研究关注运动干预下的肠道微生物群。此外，肠道微生物群携带的抗生素抗性基因（ARGs）可能会持续对人类健康构成威胁。在此，利用16S rRNA基因测序和高通量定量PCR技术对中国和巴基斯坦参与者的ARGs和微生物群进行了研究。在观察期内，运动对所调查的12名个体的肠道微生物群没有影响，而在不同国籍人群中发现了肠道微生物群的不同分布。总体而言，参与者肠道中的优势微生物门为拟杆菌门、厚壁菌门和变形菌门。一些属，如[此处原文缺失具体属名]在巴基斯坦参与者中更为丰富，而其他一些属，如[此处原文缺失具体属名]在中国参与者中更为丰富。中国人的微生物多样性高于巴基斯坦人。此外，中国和巴基斯坦人的微生物群落结构也不同。对于ARGs，在每个时间点所有检测到的ARGs的分布没有明显差异。在这些ARGs中，[此处原文缺失具体ARGs名称]在中国和巴基斯坦参与者中的分布不同，一些ARGs，如[此处原文缺失具体ARGs名称]与几种优势微生物群呈正相关，特别是随时间没有波动的拟杆菌门和厚壁菌门细菌。
