Bandeira Marina, Chee Bor Shin, Frassini Rafaele, Nugent Michael, Giovanela Marcelo, Roesch-Ely Mariana, Crespo Janaina da Silva, Devine Declan M
Materials Research Institute, Athlone Institute of Technology, N37 HD68 Athlone, Ireland.
Área do Conhecimento de Ciências Exatas e Engenharias, Universidade de Caxias do Sul, Rua Francisco Getúlio Vargas, 1130, Caxias do Sul 95070-560, RS, Brazil.
Materials (Basel). 2021 May 27;14(11):2889. doi: 10.3390/ma14112889.
Wound infections are the main complication when treating skin wounds. This work reports a novel antimicrobial material using green synthesized zinc oxide nanoparticles (ZnONPs) incorporated in polymeric fibers for wound healing purposes. ZnONPs are a promising antimicrobial nanomaterial with high activity against a range of microorganisms, including drug-resistant bacteria. The electrospun fibers were obtained using polyacrylic acid (PAA) and polyallylamine hydrochloride (PAH) and were loaded with ZnONPs green synthesized from leaves with a spherical shape and ~18 nm diameter size. The fibers were produced using the electrospinning technique and SEM images showed a uniform morphology with a diameter of ~230 nm. EDS analysis proved a consistent dispersion of Zn in the fiber mat, however, particle agglomerates with varying sizes were observed. FTIR spectra confirmed the interaction of PAA carboxylic groups with the amine of PAH molecules. Although ZnONPs presented higher antimicrobial activity against than , resazurin viability assay revealed that the PAA/PAH/ZnONPs composite successfully inhibited both bacteria strains growth. Photomicrographs support these results where bacteria clusters were observed only in the control samples. The PAA/PAH/ZnONPs composite developed presents antimicrobial activity and mimics the extracellular matrix morphology of skin tissue, showing potential for wound healing treatments.
伤口感染是治疗皮肤伤口时的主要并发症。这项工作报道了一种新型抗菌材料，它将绿色合成的氧化锌纳米颗粒（ZnONPs）掺入聚合物纤维中用于伤口愈合。ZnONPs是一种很有前景的抗菌纳米材料，对包括耐药细菌在内的多种微生物具有高活性。通过使用聚丙烯酸（PAA）和聚烯丙胺盐酸盐（PAH）获得了电纺纤维，并负载了从树叶中绿色合成的ZnONPs，其呈球形，直径约为18nm。纤维采用静电纺丝技术制备，扫描电子显微镜图像显示其形态均匀，直径约为230nm。能谱分析证明Zn在纤维毡中分散均匀，然而，观察到了不同尺寸的颗粒团聚物。傅里叶变换红外光谱证实了PAA羧基与PAH分子胺基之间的相互作用。尽管ZnONPs对[具体细菌1]的抗菌活性高于[具体细菌2]，但刃天青活力测定表明，PAA/PAH/ZnONPs复合材料成功抑制了两种细菌菌株的生长。显微照片支持了这些结果，其中仅在对照样品中观察到细菌簇。所开发的PAA/PAH/ZnONPs复合材料具有抗菌活性，并模仿了皮肤组织的细胞外基质形态，显示出在伤口愈合治疗方面的潜力。
