Cordoba Javier, Perez Emilie, Van Vlierberghe Mick, Bertrand Amandine R, Lupo Valérian, Cardol Pierre, Baurain Denis
InBioS-PhytoSYSTEMS, Laboratoire de Génétique et Physiologie des Microalgues, ULiège, B-4000 Liège, Belgium.
InBioS-PhytoSYSTEMS, Unit of Eukaryotic Phylogenomics, ULiège, B-4000 Liège, Belgium.
Genes (Basel). 2021 May 29;12(6):842. doi: 10.3390/genes12060842.
is a well-known photosynthetic microeukaryote considered as the product of a secondary endosymbiosis between a green alga and a phagotrophic unicellular belonging to the same eukaryotic phylum as the parasitic trypanosomatids. As its nuclear genome has proven difficult to sequence, reliable transcriptomes are important for functional studies. In this work, we assembled a new consensus transcriptome by combining sequencing reads from five independent studies. Based on a detailed comparison with two previously released transcriptomes, our consensus transcriptome appears to be the most complete so far. Remapping the reads on it allowed us to compare the expression of the transcripts across multiple culture conditions at once and to infer a functionally annotated network of co-expressed genes. Although the emergence of meaningful gene clusters indicates that some biological signal lies in gene expression levels, our analyses confirm that gene regulation in euglenozoans is not primarily controlled at the transcriptional level. Regarding the origin of , we observe a heavily mixed gene ancestry, as previously reported, and rule out sequence contamination as a possible explanation for these observations. Instead, they indicate that this complex alga has evolved through a convoluted process involving much more than two partners.
是一种著名的光合微真核生物，被认为是绿藻与一种吞噬性单细胞生物之间二次内共生的产物，该吞噬性单细胞生物与寄生锥虫属于同一真核生物门。由于其核基因组已被证明难以测序，可靠的转录组对于功能研究很重要。在这项工作中，我们通过整合来自五项独立研究的测序读数，组装了一个新的共有转录组。基于与两个先前发布的转录组的详细比较，我们的共有转录组似乎是迄今为止最完整的。将读数重新映射到该转录组上使我们能够同时比较多种培养条件下转录本的表达，并推断出一个功能注释的共表达基因网络。尽管有意义的基因簇的出现表明某些生物信号存在于基因表达水平中，但我们的分析证实，眼虫类的基因调控并非主要在转录水平上受到控制。关于的起源，正如先前报道的那样，我们观察到基因谱系严重混合，并排除了序列污染作为这些观察结果的可能解释。相反，它们表明这种复杂的藻类是通过一个复杂的过程进化而来的，这个过程涉及的远不止两个伙伴。
