Mondal Shakhinur Islam, Akter Arzuba, Draper Lorraine A, Ross R Paul, Hill Colin
APC Microbiome Ireland, University College Cork, T12 YT20 Cork, Ireland.
Genetic Engineering and Biotechnology Department, Shahjalal University of Science and Technology, Sylhet 3114, Bangladesh.
Int J Mol Sci. 2021 May 26;22(11):5690. doi: 10.3390/ijms22115690.
is a spore-forming enteric pathogen causing life-threatening diarrhoea and colitis. Microbial disruption caused by antibiotics has been linked with susceptibility to, and transmission and relapse of, infection. Therefore, there is an urgent need for novel therapeutics that are effective in preventing growth, spore germination, and outgrowth. In recent years bacteriophage-derived endolysins and their derivatives show promise as a novel class of antibacterial agents. In this study, we recombinantly expressed and characterized a cell wall hydrolase (CWH) lysin from phage, phiMMP01. The full-length CWH displayed lytic activity against selected strains. However, removing the N-terminal cell wall binding domain, creating CWH, resulted in increased and/or an expanded lytic spectrum of activity. specificity was retained versus commensal clostridia and other bacterial species. As expected, the putative cell wall binding domain, CWH, was completely inactive. We also observe the effect of CWH on preventing spore outgrowth. Our results suggest that CWH has therapeutic potential as an antimicrobial agent against infection.
是一种形成芽孢的肠道病原体，可导致危及生命的腹泻和结肠炎。抗生素引起的微生物破坏与感染的易感性、传播及复发有关。因此，迫切需要有效的新型疗法来预防其生长、芽孢萌发和生长。近年来，噬菌体来源的内溶素及其衍生物有望成为一类新型抗菌剂。在本研究中，我们重组表达并鉴定了来自噬菌体phiMMP01的细胞壁水解酶（CWH）溶素。全长CWH对选定的菌株显示出裂解活性。然而，去除N端细胞壁结合结构域，产生CWH，导致裂解活性谱增加和/或扩大。与共生梭菌和其他细菌种类相比，其特异性得以保留。正如预期的那样，推定的细胞壁结合结构域CWH完全无活性。我们还观察了CWH对预防芽孢生长的作用。我们的结果表明，CWH作为抗感染的抗菌剂具有治疗潜力。
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