Shang Xiao-Fei, Dai Li-Xia, Zhang Zhi-Jun, Yang Cheng-Jie, Du Sha-Sha, Wu Tian-Lin, He Ying-Hui, Zhu Jia-Kai, Liu Ying-Qian, Yan Yin-Fang, Miao Xiao-Lou, Zhang Ji-Yu
School of Pharmacy, Lanzhou University, 222 South Tianshui Road, Lanzhou 730000, P.R. China.
Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, 335 Jiangouyan, Lanzhou 730050, P.R. China.
J Agric Food Chem. 2021 Jun 16;69(23):6455-6464. doi: 10.1021/acs.jafc.1c01385. Epub 2021 Jun 2.
causes serious plant diseases. Neocryptolepine presented the significant antifungal activity against . , however the mode of action is unclear. In this paper, we investigated the potential mode of action of neocryptolepine against . integrated the proteomics and transcriptomics. Results showed that after treatment with neocryptolepine, 1012 differentially expressed proteins and 10 920 differentially expressed genes of . were found, most of them were enriched in mitochondrial respiratory chain. It affected oxidative phosphorylation led to the enrichment of ROS and the decrease of MMP, and inhibited complex III activity with the inhibition rate of 63.51% at 10 μg/mL. The mitochondrial structural and function were damaged. Cytochrome -c1 complex subunit Rieske (UQCRFS1) with the high binding score to neocryptolepine was found as a potential target. In addition, it inhibited the sclerotia formation and presented antifungal efficacy by decreasing the diameter of a wound in potato in a concentration-dependent manner. Above results indicated that neocryptolepine inhibited the complex III activity by binding UQCRFS1 and blocked the ion transfer to cause the death of . mycelia. This study laid the foundation for the future development of neocryptolepine as an alternative biofungicide.
引起严重的植物病害。新隐丹参酮对……具有显著的抗真菌活性，然而其作用模式尚不清楚。在本文中，我们通过整合蛋白质组学和转录组学研究了新隐丹参酮对……的潜在作用模式。结果表明，用新隐丹参酮处理后，发现……有1012个差异表达蛋白和10920个差异表达基因，其中大部分富集于线粒体呼吸链。它影响氧化磷酸化，导致活性氧的富集和线粒体膜电位的降低，并抑制复合物III的活性，在10μg/mL时抑制率为63.51%。线粒体的结构和功能受到损害。发现与新隐丹参酮结合分数高的细胞色素-c1复合物亚基里斯克铁硫蛋白（UQCRFS1）作为潜在靶点。此外，它抑制菌核形成，并通过以浓度依赖的方式减小马铃薯伤口直径呈现抗真菌功效。上述结果表明，新隐丹参酮通过结合UQCRFS1抑制复合物III的活性，并阻断离子转移导致……菌丝体死亡。本研究为新隐丹参酮作为替代生物杀菌剂的未来开发奠定了基础。
