CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
Biofabrication. 2021 May 28;13(3). doi: 10.1088/1758-5090/abfd79.
The design of dynamically crosslinked hydrogel bioinks for three-dimensional (3D) bioprinting is emerging as a valuable strategy to advance the fabrication of mechanically tuneable cell-laden constructs for 3Ddisease modelling and tissue engineering applications. Herein, a dynamic bioink comprising boronic acid-functionalised laminarin and alginate is explored for bioprinting 3D constructs under physiologically relevant conditions. The formulated bioink takes advantage of a double crosslinked network that combines covalent but reversible boronate ester bonds and ionic gelation via divalent cations. Moreover, it exhibits suitable rheological properties and improved mechanical features owing to its modular crosslinking chemistry, yielding stable constructs with user-programmable architecture. We explored such dynamic bioink as a supporting matrix for different cell classes, namely osteoblast precursors, fibroblasts and breast cancer cells. The resulting cell-laden bioprinted hydrogels display a homogeneous cell distribution post-printing and exceptional cell viability (>90%) that can be maintained for prolonged time periods in culture (14 days) for all cell lines. This simple and chemically versatile approach is envisaged to accelerate the development of multifunctional bioinks and contribute towards the fabrication of biomimetic 3D scaffolds with applicability in a wide range of predictive or exploratory biomedical platforms.
用于三维（3D）生物打印的动态交联水凝胶生物墨水的设计正成为一种有价值的策略，可以推进用于 3D 疾病建模和组织工程应用的机械可调细胞载体构建体的制造。在此，探索了一种由硼酸功能化岩藻聚糖和藻酸盐组成的动态生物墨水，以在生理相关条件下进行 3D 构建体的生物打印。所配制的生物墨水利用了双重交联网络，该网络结合了共价但可还原的硼酸酯键和通过二价阳离子的离子凝胶化。此外，由于其模块化交联化学，它表现出合适的流变性能和改善的机械性能，从而产生具有用户可编程结构的稳定构建体。我们探索了这种动态生物墨水作为不同细胞类别的支撑基质，即成骨前体细胞、成纤维细胞和乳腺癌细胞。所得的细胞负载的生物打印水凝胶在打印后显示出均匀的细胞分布和出色的细胞活力（>90%），所有细胞系在培养中（14 天）都可以长时间保持活力。这种简单且化学多功能的方法有望加速多功能生物墨水的发展，并有助于制造具有广泛应用前景的仿生 3D 支架的生物模拟平台。
