Universidade Federal da Grande Dourados - UFGD, Departamento de Ciências Agrárias, Dourados, MS, Brasil.
Universidade Federal de Mato Grosso do Sul - UFMS, Programa de Pós-Graduação em Biologia Vegetal, Campo Grande, MS, Brasil.
Braz J Biol. 2021 May 28;82:e231957. doi: 10.1590/1519-6984.231957. eCollection 2021.
Essential oils from the stems and leaves of Croton doctoris were analyzed by gas chromatography and mass spectrometry, resulting in 22 identified compounds. The effects of these essential oils on the germination, root and shoot growth, total chlorophyll content, potential root respiration, peroxidase activity, catalase, superoxide dismutase, and mitotic index in lettuce and onion were determined. Antioxidant, antimicrobial, and cytotoxic activity were also investigated. The results revealed that the stem oil consisted of 15 compounds, of which caryophyllene oxide (24.5%) and E-caryophyllene (13.3%) were the major constituents. The leaf oil contained E-caryophyllene (39.6%) and α-humulene (13.2%) as major compounds. The oils inhibited the germination and growth of lettuce and onion seedlings and reduced chlorophyll content, root respiration, and cell division. They also caused oxidative stress, indicated by the increased activity of the evaluated antioxidant enzymes. These abnormal physiological processes contributed to the inhibition of plant growth. The most pronounced phytotoxic effects were observed in the stem oil. The cytotoxicity tests indicated that leaf oil was more active than stem oil, resulting from the presence of biologically active sesquiterpenes that inhibit the growth of cancer cells.
从巴豆茎和叶中提取的精油，采用气相色谱-质谱联用技术进行分析，共鉴定出 22 种化合物。这些精油对生菜和洋葱的萌发、根和芽的生长、总叶绿素含量、潜在根呼吸、过氧化物酶活性、过氧化氢酶、超氧化物歧化酶和有丝分裂指数的影响进行了测定。还研究了抗氧化、抗菌和细胞毒性活性。结果表明，茎油由 15 种化合物组成，其中石竹烯氧化物（24.5%）和 E-石竹烯（13.3%）是主要成分。叶油含有 E-石竹烯（39.6%）和α-葎草烯（13.2%）作为主要化合物。这些油抑制了生菜和洋葱幼苗的萌发和生长，降低了叶绿素含量、根呼吸和细胞分裂。它们还导致了氧化应激，这可以通过评估抗氧化酶的活性增加来表明。这些异常的生理过程导致了植物生长的抑制。茎油表现出最明显的植物毒性作用。细胞毒性试验表明，叶油比茎油更活跃，这是由于存在具有生物活性的倍半萜烯，它抑制癌细胞的生长。
