Woodroffe A J, Border W A, Theofilopoulos A N, Götze O, Glassock R J, Dixon F J, Wilson C B
Kidney Int. 1977 Oct;12(4):268-78. doi: 10.1038/ki.1977.111.
A panel of three immune complex (IC) assays was used in this study to test sera from patients with glomerulonephritis (GN): the Raji cell radioimmune assay (IRCA), the radio-labeled C1q binding assay (IC1qBA), and the microcomplement consumption test (MCT). The sensitivity and specificity of each assay was evaluated in preliminary studies, and the greater sensitivity (5 to 10microgram of aggreagated human gamma-globulin (AHG) per ml of serum) and IgG specificity of the IRCA was apparent. Problems related to the preliminary heat inactivation of test sera, the interaction of C1q with substances other than IC, and the effects of suboptimal storage of test sera were experienced with the MCT and, to a lesser extent the IC1qBA. The individual reactivities of the different assays were exploited by using them in combination. Thus ICs were detected by one or more of the assays in 87% of patients with systemic lupus erythematosus (SLE), 65% of patients with GN associated with other systemic diseases, and 39% of patients with primary GN. ICs were detected more frequently in patients with acute GN than chronic GN, and in patients with low serum C3, C4, and properdin factor B (C3PA) levels.
本研究使用一组三种免疫复合物（IC）检测方法来检测肾小球肾炎（GN）患者的血清：拉吉细胞放射免疫分析（IRCA）、放射性标记的C1q结合分析（IC1qBA）和微量补体消耗试验（MCT）。在初步研究中评估了每种检测方法的敏感性和特异性，IRCA具有更高的敏感性（每毫升血清中5至10微克聚合人γ球蛋白（AHG））和IgG特异性。MCT以及在较小程度上IC1qBA遇到了与检测血清的初步热灭活、C1q与IC以外物质的相互作用以及检测血清储存不当的影响相关的问题。通过联合使用不同的检测方法来利用它们各自的反应性。因此，在87%的系统性红斑狼疮（SLE）患者、65%的与其他全身性疾病相关的GN患者和39%的原发性GN患者中，通过一种或多种检测方法检测到了IC。与慢性GN患者相比，急性GN患者中更频繁地检测到IC，并且在血清C3、C4和备解素因子B（C3PA）水平较低的患者中也是如此。
