固相C1q和拉吉细胞放射免疫分析法在肾小球肾炎循环免疫复合物检测中的应用。
Application of the solid phase C1q and Raji cell radioimmune assays for the detection of circulating immune complexes in glomerulonephritis.
作者信息
Tung K S, Woodroffe A J, Ahlin T D, Williams R C, Wilson C B
出版信息
J Clin Invest. 1978 Jul;62(1):61-72. doi: 10.1172/JCI109115.
The C1q solid phase and Raji cell radioimmune assays were used to determine the frequency of detectable circulating immune complexes in patients with glomerulonephritis. In this study, 46% of 56 patients with glomerulonephritis had evidence of circulating immune complexes. More important, circulating immune complexes were associated with some, but not other, types of glomerulonephritis. Thus, immune complexes were detected in lupus glomerulonephritis (9/9 patients), rapidly progressive glomerulonephritis (5/6 patients), and acute nephritis (5/6 patients), but not in IgA-IgG glomerulonephritis (0/7 patients), or membranous glomerulonephritis (0/8 patients). The Raji cell radioimmune assay and the C1q solid phase radioimmune assay showed concordance of 79% in the detection of circulating immune complexes. Serial determinations, in general, showed either persistence of a negative or positive result of conversion of positive to negative.
采用C1q固相放射免疫分析法和Raji细胞放射免疫分析法测定肾小球肾炎患者中可检测到的循环免疫复合物的频率。在本研究中，56例肾小球肾炎患者中有46%有循环免疫复合物的证据。更重要的是，循环免疫复合物与某些类型而非其他类型的肾小球肾炎有关。因此，在狼疮性肾小球肾炎（9/9例患者）、快速进展性肾小球肾炎（5/6例患者）和急性肾炎（5/6例患者）中检测到免疫复合物，但在IgA-IgG肾小球肾炎（0/7例患者）或膜性肾小球肾炎（0/8例患者）中未检测到。Raji细胞放射免疫分析法和C1q固相放射免疫分析法在检测循环免疫复合物方面的一致性为79%。一般来说，连续测定结果要么持续为阴性，要么由阳性转为阴性。
Zotero 插件