MIR31HG 通过调控 POLDIP2 的表达水平来调节乳腺癌细胞的增殖、迁移和侵袭。
MIR31HG regulates the proliferation, migration and invasion of breast cancer by regulating the expression of POLDIP2.
机构信息
Department of Medical Oncology, Yantai Yuhuangding Hospital, Yantai, China.
出版信息
J BUON. 2021 Mar-Apr;26(2):459-465.
PURPOSE
This study aimed to explore the role of MIR31HG and its molecular mechanism in breast cancer (BC).
METHODS
The levels of MIR31HG in BC tissues and cell lines were detected. The correlations of MIR31HG expression level with clinical characteristics and prognosis of patients were analyzed. Then we detected the effects of MIR31HG on the proliferative, migrative and invasive abilities of BC cells. Subsequently, we detected the level of the predicted target POLDIP2 in BC. Furthermore, the effect of POLDIP2 on the malignant phenotype of MIR31HG-mediated BC was evaluated through recovery experiments.
RESULTS
MIR31HG was aberrantly up-regulated in BC tissues and cell lines, and its level was in correlation with the patient's tumor diameter, tumor node metastasis (TNM) stage, lymph node metastasis as well as the overall survival rate. Besides, MIR31HG knockdown was able to inhibit the proliferative ability, migration and invasion of BC cells. Besides, POLDIP2 was aberrantly up-regulated in BC tissues, and its expression level was in positive correlation with the level of MIR31HG. Besides, POLDIP2 overexpression could partially inhibit the proliferative, migrative and invasive abilities of BC cells. Long non-coding (lnc)RNA MIR31HG was aberrantly up-regulated in BC and its expression was associated with poor prognosis of BC patients. Additionally, the levels of MIR31HG and POLDIP2 were positively correlated.
CONCLUSIONS
The low expression of MIR31HG or POLDIP2 can inhibit the proliferative, migrative and invasive abilities of BC cells, which provides a new target for the diagnosis along with the treatment of BC.
目的
本研究旨在探讨 MIR31HG 在乳腺癌（BC）中的作用及其分子机制。
方法
检测 BC 组织和细胞系中 MIR31HG 的水平。分析 MIR31HG 表达水平与患者临床特征和预后的相关性。然后，我们检测了 MIR31HG 对 BC 细胞增殖、迁移和侵袭能力的影响。随后，我们检测了 BC 中预测靶点 POLDIP2 的水平。此外，通过恢复实验评估了 POLDIP2 对 MIR31HG 介导的 BC 恶性表型的影响。
结果
MIR31HG 在 BC 组织和细胞系中异常上调，其水平与患者肿瘤直径、肿瘤淋巴结转移（TNM）分期、淋巴结转移以及总生存率相关。此外，MIR31HG 敲低能够抑制 BC 细胞的增殖能力、迁移和侵袭。此外，POLDIP2 在 BC 组织中异常上调，其表达水平与 MIR31HG 的水平呈正相关。此外，POLDIP2 的过表达能够部分抑制 BC 细胞的增殖、迁移和侵袭能力。长链非编码（lnc）RNA MIR31HG 在 BC 中异常上调，其表达与 BC 患者的预后不良相关。此外，MIR31HG 和 POLDIP2 的水平呈正相关。
结论
MIR31HG 或 POLDIP2 的低表达可以抑制 BC 细胞的增殖、迁移和侵袭能力，为 BC 的诊断和治疗提供了新的靶点。
Zotero 插件