Department of Dermatology, People's Hospital of Juye County, Heze, China.
Eur Rev Med Pharmacol Sci. 2020 Apr;24(8):4389-4395. doi: 10.26355/eurrev_202004_21020.
The aim of this study was to explore the expression of long non-coding RNA (lncRNA) MIR31HG in malignant melanoma (MM), and to investigate its clinical significance.
The quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to detect the expression of lncRNA MIR31HG in MM tissues and cells. The relationship between lncRNA MIR31HG expression and the clinicopathological characteristics was analyzed. Furthermore, the cell counting kit-8 (CCK-8) and the transwell assays were performed to assess the effect of MIR31HG on cell proliferation and metastasis in vitro, respectively.
The expression of MIR31HG was significantly upregulated in MM tissues and cells. To explore the relationship between MIR31HG expression and clinical features, the patients were divided into two groups according to the mean expression of MIR31HG, including high expression group and low expression group. The subsequent results indicated that MIR31HG expression was correlated with lymph nodes metastasis, distal metastasis, and TNM stage. The multivariate analysis indicated that a high expression of MIR31HG could be used as an independent prognostic factor for MM. MIR31HG low-expression cells were constructed in vitro. Compared with the control cells, the cells with low expression of MIR31HG showed significantly low malignancy, including decreased cell proliferation rate and migration and invasion rates.
LncRNA MIR31HG was a novel factor involved in MM progression, which could be used as a potential biomarker and therapeutic target for MM.
本研究旨在探讨长链非编码 RNA（lncRNA）MIR31HG 在恶性黑色素瘤（MM）中的表达，并探讨其临床意义。
采用实时荧光定量聚合酶链反应（qRT-PCR）检测 MM 组织和细胞中 lncRNA MIR31HG 的表达，分析 lncRNA MIR31HG 表达与临床病理特征的关系。进一步采用细胞计数试剂盒-8（CCK-8）和 Transwell 检测，分别评估 MIR31HG 对体外细胞增殖和转移的影响。
MIR31HG 在 MM 组织和细胞中表达明显上调。为探讨 MIR31HG 表达与临床特征的关系，根据 MIR31HG 的平均表达将患者分为两组，包括高表达组和低表达组。进一步的结果表明，MIR31HG 表达与淋巴结转移、远处转移和 TNM 分期有关。多因素分析表明，MIR31HG 高表达可作为 MM 的独立预后因素。体外构建 MIR31HG 低表达细胞。与对照组细胞相比，MIR31HG 低表达细胞的恶性程度明显降低，包括增殖率、迁移和侵袭率降低。
lncRNA MIR31HG 是 MM 进展过程中的一个新的因素，可作为 MM 的潜在生物标志物和治疗靶点。
