Graduate Program in Genetics, Conservation, and Evolutionary Biology - PPG - GCBEv, Manaus, Amazonas, Brazil.
Coordination of Society, Environment and Health - COSAS; National Institute of Amazonian Research -INPA, Manaus, Amazonas, Brazil.
Med Vet Entomol. 2021 Dec;35(4):556-566. doi: 10.1111/mve.12533. Epub 2021 Jun 2.
Dillapiole, extracted from Piper aduncum essential oil and its derivatives, has been shown to be a potential alternative to the control of Aedes aegypti, which has become resistant to synthetic insecticides. Methyl ether dillapiole (MED) and temephos (TM) were compared to complement the data on the genotoxicity and developmental changes of Ae. aegypti. Over four generations (G -G ), third stage larvae were treated with MED at 60, 80 and 100 μg/mL and TM at 0.002, 0.005 and 0.007 μg/mL for 4 h. Adult females were separated to estimate oviposition and hatching rates, and total egg length. Over the four generations, a significant reduction was recorded in oviposition and hatching rates, and in mean egg length (Tukey, P < 0.05), compared with the negative control (NC). Cytological slide preparations were done from adult oocytes and larval neuroblasts. The cumulative effects of genotoxic (bridges, budding and nuclear fragmentation) and mutagenic (micronucleus and chromosomal breakage) damage was observed in the neuroblasts and oocytes of exposed mosquitoes. Developmental changes and damage to the genome of MED-treated Ae. aegypti were greater than those caused by TM. Further studies should focus on understanding the effects of the MED molecule on Ae. aegypti.
从胡椒中提取的紫堇醇，及其衍生物，已被证明是控制埃及伊蚊的一种潜在替代品，埃及伊蚊已经对合成杀虫剂产生了抗药性。比较了甲基醚紫堇醇（MED）和涕灭威（TM），以补充有关埃及伊蚊的遗传毒性和发育变化的数据。在四代（G-G）中，用 MED 在 60、80 和 100μg/mL 以及 TM 在 0.002、0.005 和 0.007μg/mL 下处理第三期幼虫 4 小时。分离成年雌性以估计产卵率和孵化率以及总卵长。与阴性对照（NC）相比，在四代中，产卵率和孵化率以及平均卵长显著降低（Tukey，P<0.05）。从成年卵母细胞和幼虫神经母细胞中制备细胞学载玻片。在暴露于 MED 的蚊子的神经母细胞和卵母细胞中观察到遗传毒性（桥、芽生和核碎片）和诱变（微核和染色体断裂）损伤的累积效应。MED 处理的埃及伊蚊的发育变化和基因组损伤大于 TM 引起的损伤。进一步的研究应该集中在理解 MED 分子对埃及伊蚊的影响。
