School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
Bioorg Med Chem Lett. 2021 Aug 15;46:128159. doi: 10.1016/j.bmcl.2021.128159. Epub 2021 May 30.
In recent years, combination therapy has gradually become one of the hot spots. As a new therapy strategy, we investigated the combination treatment of polysaccharide from Lachnum sp. (LEP-2b) with silymarin and compared the effects with mono-therapy. In this study, combining high-dose LEP-2b with silymarin (CH) significantly reduced serum biochemistry indexes (ALT, AST, AKP, LDH), hepatic inflammation (TNF-α, IL-6 and IL-1β) and improved the antioxidant status (SOD, CAT, GSH-Px, GSH, MDA and T-AOC), in which its effect on TNF-α was very significant (P < 0.001). Therefore, the expressions of related proteins in the JNK/p38 signaling pathway associated with TNF-α were examined. The result showed that CH treatment markedly increased the expression of p-p38 and inhibited the JNK phosphorylation. TUNEL staining, immunohistochemical staining and western blot assays demonstrated that the hepatoprotective effect of CH treatment was probably related with inhibiting hepatocyte apoptosis. In summary, combination of high dose LEP-2b with silymarin would be a more effective method to protect liver injury than mono-therapy.
近年来，联合治疗逐渐成为热点之一。作为一种新的治疗策略，我们研究了 Lachnum sp. 多糖（LEP-2b）与水飞蓟宾联合治疗，并将其与单药治疗进行了比较。在这项研究中，高剂量 LEP-2b 与水飞蓟宾（CH）联合使用可显著降低血清生化指标（ALT、AST、AKP、LDH）、肝炎症（TNF-α、IL-6 和 IL-1β）并改善抗氧化状态（SOD、CAT、GSH-Px、GSH、MDA 和 T-AOC），其中对 TNF-α 的作用非常显著（P < 0.001）。因此，检查了与 TNF-α 相关的 JNK/p38 信号通路的相关蛋白的表达。结果表明，CH 处理可显著增加 p-p38 的表达并抑制 JNK 磷酸化。TUNEL 染色、免疫组化染色和 Western blot 分析表明，CH 处理的肝保护作用可能与抑制肝细胞凋亡有关。综上所述，高剂量 LEP-2b 与水飞蓟宾联合治疗比单药治疗更能有效保护肝损伤。
