Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, 400067, People's Republic of China.
Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing, 400067, People's Republic of China.
Drug Des Devel Ther. 2021 May 4;15:1903-1914. doi: 10.2147/DDDT.S305033. eCollection 2021.
Silymarin contains various flavonoids and exhibits antioxidative, anti-inflammatory, and anticancer effects, in addition to other pharmacological properties. This study explored the alleviating effect of silymarin on multiple-organ damage induced by D-galactose/lipopolysaccharide in Kunming mice.
Kunming mice were injected intraperitoneally with D-galactose (30 mg/kg·BW)/LPS (3 μg/kg·BW) and then treated using silymarin with different doses (75 mg/kg·bw and 150 mg/kg·bw) via intragastric administration. Changes in organ indexes, pathological changes, liver-function index, biochemical indexes, molecular biological indexes, and genes related to the oxidation and inflammation of main organs were evaluated.
After the mice were treated with silymarin, their body weight showed no significant change, and the liver, kidney, and lung indexes of the treated mice were higher than those of the model group; meanwhile, the corresponding histopathological formation was reduced. Compared with the model group, the silymarin-treated group showed reductions in ALT, AST, and liver function indexes in the mouse serum. Silymarin treatment also increased the SOD, CAT, GSH, GSH-Px, T-AOC, IL-10, and IL-12 levels, as well as reduced the MDA, NO, IL-6, IL-1β, TNF-α, IFN-γ levels in the mouse serum and liver tissues. In addition, quantitative polymerase chain reaction analysis indicated that the mRNA expression levels of , and were higher in the liver tissue of the silymarin-treated mice than in those of the model group; meanwhile, the mRNA expression levels of , and were lower than those in the model group.
Silymarin, which exhibits antioxidative and anti-inflammatory effects, can alleviate the liver, lung, and kidney damage induced by D-galactose/lipopolysaccharide. High-dose (150 mg/kg·bw) silymarin can more effectively inhibit organ damage, compared with low-dose silymarin (75 mg/kg·bw) in Kunming mice.
水飞蓟素含有多种类黄酮，具有抗氧化、抗炎和抗癌等多种药理作用。本研究探讨了水飞蓟素对 D-半乳糖/脂多糖诱导的昆明小鼠多器官损伤的缓解作用。
昆明小鼠腹腔注射 D-半乳糖（30mg/kg·BW）/LPS（3μg/kg·BW），然后用不同剂量（75mg/kg·bw 和 150mg/kg·bw）的水飞蓟素进行灌胃处理。评估器官指数、病理变化、肝功能指数、生化指标、分子生物学指标以及与主要器官氧化和炎症相关的基因的变化。
用水飞蓟素处理后，小鼠体重无明显变化，处理组小鼠的肝、肾、肺指数均高于模型组；同时，相应的组织病理学形态减少。与模型组相比，水飞蓟素处理组小鼠血清 ALT、AST 和肝功能指数降低。水飞蓟素处理还增加了 SOD、CAT、GSH、GSH-Px、T-AOC、IL-10 和 IL-12 水平，降低了 MDA、NO、IL-6、IL-1β、TNF-α和 IFN-γ水平在小鼠血清和肝组织中。此外，定量聚合酶链反应分析表明，水飞蓟素处理组小鼠肝组织中 、 和 的 mRNA 表达水平高于模型组；同时， 、 和 的 mRNA 表达水平低于模型组。
水飞蓟素具有抗氧化和抗炎作用，可缓解 D-半乳糖/脂多糖诱导的肝、肺和肾损伤。与低剂量（75mg/kg·bw）相比，高剂量（150mg/kg·bw）水飞蓟素更能有效抑制昆明小鼠的器官损伤。
