COPD 合并 T2DM 患者中辅助性 T 细胞 17/调节性 T 细胞失衡。
T-Helper 17 Cell/Regulatory T-Cell Imbalance in COPD Combined with T2DM Patients.
机构信息
Second Department of Respiratory Medicine, The First Affiliated Hospital of Kunming Medical University, Kunming, 650032, People's Republic of China.
Department of Burns Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, 650101, People's Republic of China.
出版信息
Int J Chron Obstruct Pulmon Dis. 2021 May 27;16:1425-1435. doi: 10.2147/COPD.S306406. eCollection 2021.
BACKGROUND
Chronic obstructive pulmonary disease (COPD) is often combined with type 2 diabetes mellitus (T2DM) in clinical, and with poor prognosis. In recent years, research shows that inflammation is a common characteristic of COPD and T2DM. T-helper 17 cell (Th17)/regulatory T-cell (Treg) balance controls inflammation and may be important in the pathogenesis of COPD combined with T2DM patients. This study investigated the characteristics of Th17, Treg and related inflammatory factors in COPD combined with T2DM patients and the potential mechanism.
METHODS
Application of flow cytometry technology, real-time fluorescent quantitative PCR and ELISA to detect the changes in peripheral blood of Th17 and Treg number and the expression of key transcription factors and related cytokines in COPD combined T2DM patients were performed.
RESULTS
Patients with COPD combined with T2DM revealed significant increase in peripheral Th17, Th17 related cytokines (IL-17A, IL-17F, IL-21, IL-23, IL-6) and transcription factor (RORγt) levels and significant decrease in Treg, Treg-related cytokines (IL-10, TGFβ1) and transcription factor (Foxp3) as compared with patients with COPD, T2DM and healthy controls.
CONCLUSION
Th17/Treg functional imbalance exists in patients with COPD combined with T2DM, indicating a potential role of Th17/Treg imbalance in the formation and progression of COPD combined with T2DM.
背景
慢性阻塞性肺疾病（COPD）常与 2 型糖尿病（T2DM）合并存在于临床中，并具有较差的预后。近年来，研究表明炎症是 COPD 和 T2DM 的共同特征。辅助性 T 细胞 17（Th17）/调节性 T 细胞（Treg）平衡控制炎症，可能在 COPD 合并 T2DM 患者的发病机制中具有重要作用。本研究旨在探讨 COPD 合并 T2DM 患者中 Th17、Treg 及其相关炎症因子的特征及其潜在机制。
方法
采用流式细胞术技术、实时荧光定量 PCR 和 ELISA 检测 COPD 合并 T2DM 患者外周血 Th17、Treg 数量及其关键转录因子和相关细胞因子的变化。
结果
与 COPD 患者、T2DM 患者和健康对照组相比，COPD 合并 T2DM 患者外周血 Th17、Th17 相关细胞因子（IL-17A、IL-17F、IL-21、IL-23、IL-6）和转录因子（RORγt）水平显著升高，Treg、Treg 相关细胞因子（IL-10、TGFβ1）和转录因子（Foxp3）水平显著降低。
结论
COPD 合并 T2DM 患者存在 Th17/Treg 功能失衡，提示 Th17/Treg 失衡在 COPD 合并 T2DM 的形成和进展中可能具有潜在作用。
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