State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P. R. China.
Langmuir. 2021 Jun 15;37(23):7264-7272. doi: 10.1021/acs.langmuir.1c01020. Epub 2021 Jun 3.
Developing powerful real-time methods for monitoring the thrombolytic process is highly desirable for the early therapy of thrombus diseases. Herein, an optical interference fibrin was constructed, fabricated by assembling a 190 nm silica colloidal crystal on glass slides, for detecting a thrombolytic process through the shift of interference peaks caused by the variation of the thicknesses of a silica colloidal crystal film with loaded fibrin dissolution. The whole kinetic progress of thrombolysis by nattokinase and urokinase as thrombolytic drug models was recorded, and the kinetic data were calculated. Moreover, the developed method shows excellent sensitivity for the activity of nattokinase and urokinase with wide linear ranges of approximately 0.75-750 and 5-1000 units mL, respectively. Thus, this method can be used as a real-time, low-cost, and simple system for monitoring the thrombolytic process of drugs, demonstrating huge potential in the development of treating thromboembolic diseases and screening drugs.
开发强大的实时方法来监测溶栓过程对于血栓疾病的早期治疗非常重要。在此，构建了一种光学干涉纤维蛋白，通过组装在玻璃载玻片上的 190nm 二氧化硅胶体晶体，来检测溶栓过程，其通过负载纤维蛋白溶解引起的二氧化硅胶体晶体膜厚度变化引起的干涉峰的移动来实现。记录了纳豆激酶和尿激酶作为溶栓药物模型的溶栓的整个动力学过程，并计算了动力学数据。此外，所开发的方法对纳豆激酶和尿激酶的活性具有优异的灵敏度，其线性范围分别约为 750 至 750 单位/mL 和 5 至 1000 单位/mL。因此，该方法可以用作实时、低成本和简单的药物溶栓过程监测系统，在开发治疗血栓栓塞性疾病和筛选药物方面具有巨大的潜力。
