Department of Neurology, The Affiliated WuXi No.2 People's Hospital of Nanjing Medical University, Wuxi, China.
Department of Neurology, the WuXi NO.2 People's Hospital, Affiliated Wuxi Clinical College of Nantong University, Wuxi, Jiangsu, China.
Am J Alzheimers Dis Other Demen. 2021 Jan-Dec;36:15333175211021712. doi: 10.1177/15333175211021712.
This paper was aimed to analyze the microRNA (miRNA) signatures in Alzheimer disease (AD) and find the significant expressions of miRNAs, their target genes, the functional enrichment analysis of the confirmed genes, and potential drug treatment. The miRNA expression information of the gene expression profile data was downloaded from the Gene Expression Omnibus database. The total data sample size is 1309, including 1021 AD samples and 288 normal samples. A total of 21 differentially expressed miRNAs were obtained, of which 16 (hsa-miR-6761-3p, hsa-miR-6747-3p, hsa-miR-6875-3p, hsa-miR-6754-3p, hsa-miR-6736-3p, hsa-miR-6762-3p, hsa-miR-6787-3p, hsa-miR-208a-5p, hsa-miR-6740-3p, hsa-miR-6778-3p, hsa-miR-595, hsa-miR-6753-3p, hsa-miR-4747-3p, hsa-miR-3646, hsa-miR-6716-3p and hsa-miR-4435) were up-regulated and 5 (hsa-miR-125a-3p, hsa-miR-22-3p, hsa-miR-24-3p, hsa-miR-6131 and hsa-miR-125b-1-3p) were down-regulated in AD. A total of 6 miRNAs (hsa-miR-595, hsa-miR-3646, hsa-miR-4435 hsa-miR-125a-3p, hsa-miR-22-3p and hsa-miR-24-3p) and 78 miRNA-disease-related gene sub-networks were predicted, and 116 ceRNA regulatory relationship pairs, and the ceRNA regulatory network were obtained. The results of enrichment analysis suggested that the main target pathways of several miRNAs differentially expressed in AD were mitogen-activated protein kinase signal pathway. According to the prediction results of Drug-Gene Interaction database 2.0, we obtained 53 pairs of drug-gene interaction, including 7 genes (PTGS2, EGFR, CALM1, PDE4D, FGFR2, HMGCR, cdk6) and 53 drugs. We hope our results are helpful to find a viable way to prevent, delay the onset, diagnose, and treat AD.
本文旨在分析阿尔茨海默病（AD）中的 microRNA（miRNA）特征，寻找有显著表达的 miRNAs、它们的靶基因、确认基因的功能富集分析以及潜在的药物治疗方法。从基因表达综合数据库（Gene Expression Omnibus database）中下载 miRNA 表达信息的基因表达谱数据集。总数据样本量为 1309 个，包括 1021 个 AD 样本和 288 个正常样本。共获得 21 个差异表达的 miRNAs，其中 16 个（hsa-miR-6761-3p、hsa-miR-6747-3p、hsa-miR-6875-3p、hsa-miR-6754-3p、hsa-miR-6736-3p、hsa-miR-6762-3p、hsa-miR-6787-3p、hsa-miR-208a-5p、hsa-miR-6740-3p、hsa-miR-6778-3p、hsa-miR-595、hsa-miR-6753-3p、hsa-miR-4747-3p、hsa-miR-3646、hsa-miR-6716-3p 和 hsa-miR-4435）上调，5 个（hsa-miR-125a-3p、hsa-miR-22-3p、hsa-miR-24-3p、hsa-miR-6131 和 hsa-miR-125b-1-3p）下调。共预测到 6 个 miRNA（hsa-miR-595、hsa-miR-3646、hsa-miR-4435、hsa-miR-125a-3p、hsa-miR-22-3p 和 hsa-miR-24-3p）和 78 个 miRNA-疾病相关基因亚网络，以及 116 个 ceRNA 调控关系对，并获得 ceRNA 调控网络。富集分析结果表明，AD 中几个差异表达 miRNA 的主要靶途径是有丝分裂原激活蛋白激酶信号通路。根据 Drug-Gene Interaction database 2.0 的预测结果，我们获得了 53 对药物-基因相互作用，包括 7 个基因（PTGS2、EGFR、CALM1、PDE4D、FGFR2、HMGCR、cdk6）和 53 种药物。我们希望我们的研究结果有助于找到一种可行的方法来预防、延缓 AD 的发病、诊断和治疗 AD。
