Hubei Tumor Biological Behavior Key Laboratory, Center of stomatology, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, China; Department of Prosthodontics, Hefei Stomatological Clinic Hospital, Anhui Medical University, & Hefei Stomatological Hospital, Hefei 230001, Anhui Province, China.
Hubei Tumor Biological Behavior Key Laboratory, Center of stomatology, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, China.
Mater Sci Eng C Mater Biol Appl. 2021 Jul;126:112130. doi: 10.1016/j.msec.2021.112130. Epub 2021 Apr 24.
In recent years, photocrosslinked alginate hydrogel has been widely studied in bone tissue engineering, owing to its numerous advantages. However, there are still some shortcomings like insufficient mechanical strength and lack of bone induction. To compensate for these deficiencies, in this work, a novel doped strontium (Sr) photocrosslinked methacrylated alginate (Sr-PMA) hydrogel was developed. Photocrosslinked alginate hydrogel fabricated via crosslinking methacrylate-modified alginate under ultraviolet (UV) light was placed into strontium solutions to prepare Sr-PMA gel by chelating reaction. The chemical structures, swelling behaviors, degradation profiles, elastic moduli, Sr ion release and surface morphology of the Sr-PMA hydrogel were characterized, and we found that physical properties of the gels can be tailored by varying concentration of Sr ions. And MC3T3-E1 cell viability, proliferation and mineralization outside the hydrogel were also investigated. Further research on cell survival, multiplication, osteogenic differentiation of the cells encapsulated in Sr-PMA hydrogels were explored. In vitro studies of biological properties revealed that incorporation of Sr into photocrosslinked alginate gels significantly improved osteogenic differentiation capabilities and mineralization via stimulating expression of osteogenesis related genes and proteins of the cells compared to strontium-free photocrosslinked alginate gels. The research demonstrates that the innovative Sr-PMA hydrogels possessing adjustable physical performances, excellent biocompatibility and osteogenic differentiation capabilities could be potentially applied to bone tissue engineering and regenerative medicine. Meanwhile, it also provides a reference for the modification of biological properties of biomaterials.
近年来，光交联海藻酸盐水凝胶因其诸多优点在骨组织工程中得到了广泛的研究。然而，它仍然存在一些缺点，如机械强度不足和缺乏骨诱导。为了弥补这些不足，在这项工作中，开发了一种新型掺杂锶（Sr）的光交联甲基丙烯酰化海藻酸盐（Sr-PMA）水凝胶。通过紫外（UV）光交联甲基丙烯酰化修饰的海藻酸盐制备光交联海藻酸盐水凝胶，然后将其置于锶溶液中，通过螯合反应制备 Sr-PMA 凝胶。对 Sr-PMA 水凝胶的化学结构、溶胀行为、降解特性、弹性模量、Sr 离子释放和表面形貌进行了表征，结果发现可以通过改变 Sr 离子浓度来调整水凝胶的物理性能。并且还研究了 Sr-PMA 水凝胶外的 MC3T3-E1 细胞活力、增殖和矿化情况。进一步研究了 Sr-PMA 水凝胶中细胞的存活、增殖和成骨分化情况。体外生物性能研究表明，与不含锶的光交联海藻酸盐凝胶相比，Sr 掺入光交联海藻酸盐凝胶可以通过刺激细胞表达成骨相关基因和蛋白，显著提高成骨分化能力和矿化能力。研究表明，具有可调物理性能、良好的生物相容性和成骨分化能力的新型 Sr-PMA 水凝胶可能应用于骨组织工程和再生医学。同时，它也为生物材料生物性能的修饰提供了参考。
