Nanomaterials and Nanotechnology Research Centre - CINN (CSIC), 33940, El Entrego, Asturias, Spain.
Nanomaterials and Nanotechnology Research Centre - CINN (CSIC), 33940, El Entrego, Asturias, Spain; Department of Organic and Inorganic Chemistry, University of Oviedo, 33006 Oviedo, Spain.
Mater Sci Eng C Mater Biol Appl. 2021 Jul;126:112168. doi: 10.1016/j.msec.2021.112168. Epub 2021 May 5.
Biomaterials and their surfaces regulate the biological response and ultimately the quality of healing at a possible site of implantation. The physical, chemical and topographical properties of implants' surfaces play a decisive role in the biological integration process for their immediate loading and long-term success. Since at this level of biological interaction nano-dimensionality is basically entailed, bio-functional nanostructured composites either as filling/cement or coating to metallic implants are required. This study shows the possibility of synthesizing two phases of nanostructured titanium phosphate (π and ρ polymorphs) and enriching them with silver nanoparticles and strontium. More importantly, Ag-Sr-enriched nanostructured π‑titanium phosphate is induced to grow on a commercially available titanium alloy (Ti-6Al-4V), widely used in orthopedic and dental implants, under highly controlled conditions. Structural and microscopic studies, using XRD, HRTEM and SEM altogether confirm the resultant phases and their enrichment with strontium and silver nanoparticles with an average particle size around 6 nm. Using confocal laser scanning microscopy, the surface roughness was measured and is found to lay at the interface between the nanosized and microsized topologies. Ion release assessments showed that the presence of strontium controlled the release rate of silver ions and this could be beneficial in terms of decreasing the accompanied cytotoxicity that is usually encountered at high concentrations of silver release. Antimicrobial and cell proliferation assays have proved that enriching titanium phosphate with strontium and silver nanoparticles has improved their antimicrobial properties, while the cytotoxicity could be controlled.
生物材料及其表面调节着生物反应，并最终影响植入部位的愈合质量。植入物表面的物理、化学和形貌特性在其即刻负载和长期成功的生物整合过程中起着决定性的作用。由于在这种水平的生物相互作用中基本涉及到纳米级，因此需要生物功能纳米结构复合材料作为填充/水泥或金属植入物的涂层。本研究展示了合成两种纳米结构磷酸钛（π 和 ρ 多晶型物）并对其进行银纳米粒子和锶富集的可能性。更重要的是，在高度受控的条件下，Ag-Sr 富化纳米结构 π-磷酸钛被诱导在商业上可获得的钛合金（Ti-6Al-4V）上生长，该钛合金广泛用于骨科和牙科植入物。使用 XRD、HRTEM 和 SEM 的结构和微观研究共同证实了所得相及其与锶和银纳米粒子的富集，平均粒径约为 6nm。使用共聚焦激光扫描显微镜测量表面粗糙度，发现其位于纳米级和微级形貌的界面之间。离子释放评估表明，锶的存在控制了银离子的释放速率，这在降低通常在高浓度银释放时遇到的伴随细胞毒性方面可能是有益的。抗菌和细胞增殖试验已经证明，用锶和银纳米粒子对磷酸钛进行富化，提高了其抗菌性能，同时可以控制细胞毒性。
