Laboratório de Zoonoses Bacterianas, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil.
Laboratory of Microbiology, Parasitology and Hygiene, Department of Pharmaceutical Sciences, University of Antwerp, Wilrijk, Belgium.
Rev Argent Microbiol. 2022 Apr-Jun;54(2):95-99. doi: 10.1016/j.ram.2021.03.002. Epub 2021 Jun 1.
Changes were made to the original formulation of the EMJH medium (Ellinghausen-McCullough-Johnson-Harris) enrichment and some aspects such as growth time of Leptospira and utilization in the microscopic agglutination test (MAT) were evaluated and compared to the original enrichment and to a commercially available enrichment (DIFCO™). Leptospira samples (24 antigens) that make up our panel of antigens used in MAT were used, among them, reference and autochthonous strains isolated in Brazil. The samples were grown individually in the EMJH medium under the three previously mentioned conditions (adapted enrichment, original enrichment and commercial enrichment). In addition, 89 blood serums from domestic and wild animals were analyzed by MAT using the antigens grown in these media. All samples tested grew efficiently with the adapted enrichment, and the MAT results were satisfactory. Therefore, other laboratories could also benefit from the use of this adapted enrichment when culturing the Leptospira strains applied in their MAT panels.
对 EMJH 培养基（埃利希-麦克卡洛夫-约翰逊-哈里斯）的原始配方进行了修改，并评估和比较了其在一些方面的变化，如钩端螺旋体的生长时间和在显微镜凝集试验（MAT）中的应用，与原始培养基和一种市售培养基（DIFCO™）相比。我们的 MAT 抗原板由 24 种抗原组成，其中包括在巴西分离的参考和本土菌株。这些样本在上述三种条件下（改良培养基、原始培养基和商业培养基）分别在 EMJH 培养基中培养。此外，使用这些培养基中培养的抗原，对 89 份来自家养和野生动物的血清进行了 MAT 分析。改良培养基可以有效地培养所有测试样本，MAT 结果令人满意。因此，其他实验室在培养其 MAT 板中应用的钩端螺旋体菌株时，也可以受益于使用这种改良培养基。
