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从显微镜检查到纳米显微镜检查：在纳米尺度上定义减数分裂图谱。

From Microscopy to Nanoscopy: Defining an Meiotic Atlas at the Nanometer Scale.

作者信息

Sims Jason, Schlögelhofer Peter, Kurzbauer Marie-Therese

机构信息

Department of Chromosome Biology, Max Perutz Labs, University of Vienna, Vienna BioCenter, Vienna, Austria.

出版信息

Front Plant Sci. 2021 May 18;12:672914. doi: 10.3389/fpls.2021.672914. eCollection 2021.

DOI:10.3389/fpls.2021.672914
PMID:34084178
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8167036/
Abstract

Visualization of meiotic chromosomes and the proteins involved in meiotic recombination have become essential to study meiosis in many systems including the model plant . Recent advances in super-resolution technologies changed how microscopic images are acquired and analyzed. New technologies enable observation of cells and nuclei at a nanometer scale and hold great promise to the field since they allow observing complex meiotic molecular processes with unprecedented detail. Here, we provide an overview of classical and advanced sample preparation and microscopy techniques with an updated meiotic atlas based on super-resolution microscopy. We review different techniques, focusing on stimulated emission depletion (STED) nanoscopy, to offer researchers guidance for selecting the optimal protocol and equipment to address their scientific question.

摘要

在包括模式植物在内的许多系统中，减数分裂染色体及参与减数分裂重组的蛋白质的可视化已成为研究减数分裂的关键。超分辨率技术的最新进展改变了显微图像的获取和分析方式。新技术能够在纳米尺度上观察细胞和细胞核，为该领域带来了巨大的前景，因为它们可以以前所未有的细节观察复杂的减数分裂分子过程。在此，我们基于超分辨率显微镜提供了一个更新的减数分裂图谱，概述了经典和先进的样品制备及显微镜技术。我们回顾了不同的技术，重点介绍了受激发射损耗（STED）纳米显微镜，为研究人员选择最佳方案和设备以解决其科学问题提供指导。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3954/8167036/45c1d83a08c4/fpls-12-672914-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3954/8167036/5b48791e350c/fpls-12-672914-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3954/8167036/c7279260fe28/fpls-12-672914-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3954/8167036/361f054754b8/fpls-12-672914-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3954/8167036/e41ebd963f99/fpls-12-672914-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3954/8167036/414afdb9228b/fpls-12-672914-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3954/8167036/6a2199766c92/fpls-12-672914-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3954/8167036/45c1d83a08c4/fpls-12-672914-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3954/8167036/5b48791e350c/fpls-12-672914-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3954/8167036/c7279260fe28/fpls-12-672914-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3954/8167036/361f054754b8/fpls-12-672914-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3954/8167036/e41ebd963f99/fpls-12-672914-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3954/8167036/414afdb9228b/fpls-12-672914-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3954/8167036/6a2199766c92/fpls-12-672914-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3954/8167036/45c1d83a08c4/fpls-12-672914-g007.jpg

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