Mogi Katsuo, Kimura Hiroyuki, Kondo Yuto, Inoue Tomoya, Adachi Shungo, Natsume Tohru
Cellular and Molecular Biotechnology Research Institute (CMB), National Institute of Advanced Industrial Science and Technology (AIST), Tokyo 135-0064, Japan.
Department of Analytical and Bioinorganic Chemistry, Kyoto Pharmaceutical University, Kyoto 607-8412, Japan.
R Soc Open Sci. 2021 May 26;8(5):201809. doi: 10.1098/rsos.201809.
Delicate animal experiments and microdose clinical trials using short-lived radioisotopes require rapid preparation with high accuracy and careful attention to safety within a limited timeframe. We have developed an open-style electrowetting on dielectric (EWOD) device containing dimple structures for the rapid preparation of radiolabelled reagents. The device was demonstrated by automatic preparation of a technetium diethylenetriamine pentaacetate (Tc-DTPA) with high chelation efficiency (99.7 ± 0.13%). Additionally, we demonstrated the single-photon emission computed tomography/computed tomography imaging of mouse kidney using the Tc-DTPA prepared with the EWOD device. The obtained organ tomographic images were sufficient for the evaluation of mouse models for specific diseases. These results indicate that manual radiolabelling for a small amount of nuclear medicine can be replaced by a process using the proposed EWOD device as a human error reduction technique.
使用短寿命放射性同位素进行精细的动物实验和微剂量临床试验，需要在有限的时间内进行快速且高精度的制备，并严格注意安全问题。我们开发了一种包含微凹结构的开放式介电电泳（EWOD）装置，用于快速制备放射性标记试剂。通过自动制备螯合效率高（99.7±0.13%）的锝二乙三胺五乙酸（Tc-DTPA），证明了该装置的性能。此外，我们还展示了使用EWOD装置制备的Tc-DTPA对小鼠肾脏进行的单光子发射计算机断层扫描/计算机断层扫描成像。所获得的器官断层图像足以用于评估特定疾病的小鼠模型。这些结果表明，作为一种减少人为误差的技术，使用所提出的EWOD装置的过程可以取代少量核医学的手动放射性标记。
