IGFL2-AS1 通过 Wnt/β-catenin 信号通路促进舌鳞状细胞癌的进展。
IGFL2-AS1 facilitates tongue squamous cell carcinoma progression via Wnt/β-catenin signaling pathway.
机构信息
Department of Otorhinolaryngology Head and Neck Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Department of Pediatrics, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
出版信息
Oral Dis. 2023 Mar;29(2):469-482. doi: 10.1111/odi.13935. Epub 2021 Jun 21.
OBJECTIVES
Tongue squamous cell carcinoma (TSCC) is the most common malignancy in oral cancer. Long noncoding RNAs (lncRNAs) are important regulators in cancer biology. In our present study, we investigated a novel lncRNA IGF-like family member 2 antisense RNA 1 (IGFL2-AS1) in TSCC.
METHODS
RT-qPCR analyzed IGFL2-AS1 expression in TSCC cells. Functional assays assessed the impact of IGFL2-AS1 on TSCC cell proliferation, migration, and invasion. Western blot analyzed the protein levels of EMT-related markers. Mechanism assays analyzed the regulatory mechanism of IGFL2-AS1 in TSCC cells. In-vivo experiments were conducted to prove the role of IGFL2-AS1 in TSCC progression.
RESULTS
IGFL2-AS1 was significantly up-regulated in TSCC cells and tissues, and IGFL2-AS1 knockdown inhibited cell proliferation, migration, invasion and EMT in TSCC. Moreover, IGFL2-AS1 functioned as a competing endogenous RNA (ceRNA) to sponge miR-1224-5p and thereby modulated SATB homeobox 1 (SATB1) expression. Additionally, SATB1 activated the Wnt/β-catenin signaling pathway in TSCC cells and IGFL2-AS1 regulated the Wnt/β-catenin signaling pathway and TSCC progression via elevating SATB1 expression.
CONCLUSIONS
The data revealed that IGFL2-AS1 played a cancer promoting role in TSCC and may aid in exploring a brand new biomarker that might contribute to TSCC treatment.
目的
舌鳞状细胞癌（TSCC）是口腔癌中最常见的恶性肿瘤。长链非编码 RNA（lncRNA）是癌症生物学中的重要调控因子。在本研究中，我们研究了舌鳞状细胞癌中一种新的 lncRNA IGF 样家族成员 2 反义 RNA 1（IGFL2-AS1）。
方法
RT-qPCR 分析 TSCC 细胞中 IGFL2-AS1 的表达。功能分析评估了 IGFL2-AS1 对 TSCC 细胞增殖、迁移和侵袭的影响。Western blot 分析 EMT 相关标志物的蛋白水平。机制分析评估了 IGFL2-AS1 在 TSCC 细胞中的调控机制。体内实验证明了 IGFL2-AS1 在 TSCC 进展中的作用。
结果
IGFL2-AS1 在 TSCC 细胞和组织中显著上调，IGFL2-AS1 敲低抑制 TSCC 细胞的增殖、迁移、侵袭和 EMT。此外，IGFL2-AS1 作为竞争性内源性 RNA（ceRNA）发挥作用，可吸附 miR-1224-5p，从而调节 SATB 盒 1（SATB1）的表达。此外，SATB1 在 TSCC 细胞中激活 Wnt/β-catenin 信号通路，IGFL2-AS1 通过上调 SATB1 表达调节 Wnt/β-catenin 信号通路和 TSCC 进展。
结论
数据表明，IGFL2-AS1 在 TSCC 中发挥促癌作用，可能有助于探索新的生物标志物，有助于 TSCC 的治疗。
Zotero 插件