Ma Qian, Xia Li, Tan Miao, Sun Quanwei, Yang Mengya, Zhang Ying, Chen Ning
College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
Sheng Wu Gong Cheng Xue Bao. 2021 May 25;37(5):1677-1696. doi: 10.13345/j.cjb.200588.
Fermentative production of amino acids is one of the pillars of the fermentation industry in China. Recently, with the fast development of metabolic engineering and synthetic biology technologies, the metabolic engineering for production of amino acids has been flourishing. Conventional forward metabolic engineering, reversed metabolic engineering based on omics data and in silico simulation, and evolutionary metabolic engineering mimicking the natural evolution, have shown increasingly promising applications. A series of highly efficient and robust amino acids-producing strains have been developed and applied in the industrial production of amino acids. The increasingly fierce market competition has put forward new requirements for strain breeding and selection, such as developing high value-added amino acids, dynamic regulation of cellular metabolism, and adapting to the requirements of new process. This review summarizes the advances and prospects in metabolic engineering for the production of amino acids.
氨基酸的发酵生产是中国发酵工业的支柱之一。近年来，随着代谢工程和合成生物学技术的快速发展，氨基酸生产的代谢工程蓬勃发展。传统的正向代谢工程、基于组学数据和计算机模拟的逆向代谢工程以及模拟自然进化的进化代谢工程，都显示出越来越有前景的应用。一系列高效、稳健的氨基酸生产菌株已被开发并应用于氨基酸的工业生产。日益激烈的市场竞争对菌株选育提出了新的要求，如开发高附加值氨基酸、细胞代谢的动态调控以及适应新工艺的要求等。本文综述了氨基酸生产代谢工程的研究进展与展望。
