Carlson College of Veterinary Medicine, Oregon State University, Corvallis, Oregon, United States of America.
Environmental & Fisheries Science Division, Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, Newport, Oregon, United States of America.
PLoS One. 2021 Jun 4;16(6):e0246982. doi: 10.1371/journal.pone.0246982. eCollection 2021.
Plasma biochemistry and hematology reference intervals are integral health assessment tools in all medical fields, including aquatic animal health. As sablefish (Anoplopoma fimbria) are becoming aquaculturally and economically more important, this manuscript provides essential reference intervals (RI) for their plasma biochemistry and hematology along with reference photomicrographs of blood cells in healthy, fasted sablefish. Blood cell morphology can differ between fish species. In addition, blood cell counts and blood chemistry can vary between fish species, demographics, water conditions, seasons, diets, and culture systems, which precludes the use of RI's from other fish species. For this study, blood was collected for plasma biochemistry and hematology analysis between June 20 and July 18, 2019, from healthy, yearling sablefish, hatched and reared in captivity on a commercial diet. Overnight fast of 16-18 hours did not sufficiently reduce lipids in the blood, which led to visible lipemia and frequent rupture of blood cells during analysis. Therefore, sablefish should be fasted for 24 to 36 hours before blood is collected to reduce hematology artifacts or possible reagent interference in plasma biochemistry analysis. Lymphocytes were the most dominant leukocytes (98%), while eosinophils were rare, and basophils were not detected in sablefish. Neutrophils were very large cells with Döhle bodies. In mammals and avian species, Döhle bodies are usually signs of toxic change from inflammation, but no such association was found in these fish. In conclusion, lipemia can interfere with sablefish blood analysis, and available removal methods should be evaluated as fasting for up to 36 h might not always be feasible. Also, more studies are required to establish RI for different developmental stages and rearing conditions.
血浆生化和血液学参考区间是所有医学领域（包括水生动物健康）整体健康评估工具。随着黑鮶（Anoplopoma fimbria）在水产养殖和经济方面变得越来越重要，本文提供了其血浆生化和血液学的重要参考区间（RI），以及健康禁食黑鮶血液中血细胞的参考显微照片。鱼类物种之间的血细胞形态可能存在差异。此外，血细胞计数和血液化学在鱼类物种、种群、水质条件、季节、饮食和养殖系统之间可能存在差异，这使得不能使用其他鱼类物种的 RI。在这项研究中，于 2019 年 6 月 20 日至 7 月 18 日，从在商业饮食中人工孵化和饲养的健康的一岁黑鮶中采集血液，用于进行血浆生化和血液学分析。16-18 小时的隔夜禁食并没有充分减少血液中的脂质，导致在分析过程中血液出现乳糜和血细胞频繁破裂。因此，在采集血液之前，黑鮶应该禁食 24 到 36 小时，以减少血液学伪影或可能的试剂干扰血浆生化分析。淋巴细胞是最主要的白细胞（98%），而嗜酸性粒细胞很少，在黑鮶中未检测到嗜碱性粒细胞。中性粒细胞是具有多染色性小体的大型细胞。在哺乳动物和禽类中，多染色性小体通常是炎症引起的毒性变化的标志，但在这些鱼类中没有发现这种关联。总之，脂血症会干扰黑鮶的血液分析，应评估可用的去除方法，因为长达 36 小时的禁食可能并不总是可行。此外，还需要更多的研究来建立不同发育阶段和饲养条件的 RI。
