Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.
Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Mar Environ Res. 2021 Jul;169:105377. doi: 10.1016/j.marenvres.2021.105377. Epub 2021 May 30.
Microplastics (MPs) are a new type of environmental pollutant. To investigate the response of shrimp and their microflora to MPs, Litopenaeus vannamei (L. vannamei) was exposed to different concentrations of MPs (0, 50, 500, and 5000 μg/L, i.e., C, L, M and H groups) for 48 h. The survival rate, intake of MPs, immune-related gene expression and microbial response under MP exposure were detected. The results showed that the survival rate in the H group was significantly lower than those in the C, L and M groups, while the relative expression levels of proPO, TLR and ALF in the M and H groups were significantly higher than those in the C and L groups. For the microbial response, microbial community richness in the L group was significantly decreased, while community richness and diversity in the H group were significantly increased compared with those in the C group. The relative abundances of 3, 4 and 11 taxa were significantly changed after MP treatment at the phylum, class and genus levels, respectively. The results suggested that short-term exposure to low concentrations of MPs did not cause immune defense responses or death but affected the balance of bacterial composition in shrimp. Exposure to high concentrations of MPs can induce immune responses and microbial changes and can even cause death in shrimp. These findings increase our understanding of MP impacts on aquatic organisms.
微塑料（MPs）是一种新型的环境污染物。为了研究虾及其微生物对 MPs 的反应，将凡纳滨对虾（L. vannamei）暴露于不同浓度的 MPs（0、50、500 和 5000μg/L，即 C、L、M 和 H 组）中 48 小时。检测了暴露于 MPs 下虾的存活率、MPs 摄入量、免疫相关基因表达和微生物反应。结果表明，H 组的存活率明显低于 C、L 和 M 组，而 M 和 H 组的 proPO、TLR 和 ALF 的相对表达水平明显高于 C 和 L 组。对于微生物反应，L 组的微生物群落丰富度明显降低，而 H 组的群落丰富度和多样性明显高于 C 组。在门、纲和属水平上，MP 处理后分别有 3、4 和 11 个分类群的相对丰度发生了显著变化。这些结果表明，短期暴露于低浓度的 MPs 不会引起免疫防御反应或死亡，但会影响虾体内细菌组成的平衡。暴露于高浓度的 MPs 会诱导免疫反应和微生物变化，甚至会导致虾死亡。这些发现增加了我们对 MPs 对水生生物影响的理解。
