Nanotechnology Center, Chemistry Department, Faculty of Science, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt; Institute of Nanoscience and Nanotechnology, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.
Nanotechnology Center, Chemistry Department, Faculty of Science, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Nov 15;261:120008. doi: 10.1016/j.saa.2021.120008. Epub 2021 May 26.
Self-assembly of Sofosbuvir drug (SOF) anti-hepatitis C virus (HCV) with bio-polymeric nanoparticles such as chitosan nanoparticles (Cs NPs) and polyvinyl alcohol nanoparticles (PVA NPs), the novel composites have been characterized successfully by different analysis such as Energy-dispersive X-ray spectroscopy (EDX), Scanning electron microscopy (SEM), UV-Visible spectrophotometer (UV-Vis) and Fourier Transmittance Infrared (FT-IR). The improvement of the Sofosbuvir effect as a result of loading drug on the bio-polymer NPs surface has been detected by the UV-Vis, and fluorescence spectroscopy techniques. The improvement of SOF efficiency was revealed via studying the drug release of SOF from biopolymers NPs surface at pH 7.4, UV-Vis spectra used for the releasing process. The binding constant (K) value was reported at 0.000055 and 0.3613 min for Cs and PVA NPs respectively. Also, the value of K was documented at 0.0014 and 7.16 min for Cs@SOF and PVA@SOF hybrid nanocomposite. The incorporation rate (k) of SOF on the surface of biopolymer nano molecules was calculated to be 0.00812 and 0.0165 min for Cs and PVA NPs, respectively. Besides the observed value of (n) was close to the unit 0.74 and 0.86 for Cs and PVA NPs, respectively. The SOF released from Cs NPs surface was documented at 0.09 mg after 24 h, while PVA NPs reported at 0.7 mg at the same time and the release efficiency is 56.5 and 73% for Cs@SOF and PVP@SOF, respectively. From the results, we suggest Cs/SOF and PVA/SOF hybrid nanocomposites have spectroscopic results that make them promising candidate drugs, but need to the clinical trials.
索非布韦（SOF）抗丙型肝炎病毒（HCV）药物与生物聚合物纳米粒子（如壳聚糖纳米粒子（Cs NPs）和聚乙烯醇纳米粒子（PVA NPs））的自组装，新型复合材料已通过能量色散 X 射线光谱（EDX）、扫描电子显微镜（SEM）、紫外可见分光光度计（UV-Vis）和傅里叶变换红外（FT-IR）等不同分析成功表征。通过紫外可见分光光度法和荧光光谱技术检测到负载药物后索非布韦（SOF）效果的提高。通过研究在 pH 7.4 时从生物聚合物 NPs 表面释放 SOF 的药物释放过程中的 UV-Vis 光谱，揭示了 SOF 效率的提高。报道了在 Cs 和 PVA NPs 上的结合常数（K）值分别为 0.000055 和 0.3613 min。此外，在 Cs@SOF 和 PVA@SOF 杂化纳米复合材料中，K 值分别记录为 0.0014 和 7.16 min。SOF 在生物聚合物纳米分子表面的负载速率（k）分别计算为 Cs 和 PVA NPs 为 0.00812 和 0.0165 min。此外，观察到的（n）值分别接近 Cs 和 PVA NPs 的单位值 0.74 和 0.86。Cs NPs 表面释放的 SOF 在 24 小时后记录为 0.09 mg，而 PVA NPs 则在同一时间报告为 0.7 mg，释放效率分别为 Cs@SOF 和 PVP@SOF 的 56.5%和 73%。从结果来看，我们建议 Cs/SOF 和 PVA/SOF 杂化纳米复合材料具有有前途的候选药物的光谱结果，但需要进行临床试验。
