Nanotechnology Center, Chemistry Department, Faculty of Science, Kafrelsheikh University, 33516, Egypt.
Nanotechnology Center, Chemistry Department, Faculty of Science, Kafrelsheikh University, 33516, Egypt; Institute of Nanoscience and Nanotechnology, Kafrelsheikh University, 33516, Egypt.
Int J Biol Macromol. 2021 Nov 1;190:927-939. doi: 10.1016/j.ijbiomac.2021.08.200. Epub 2021 Sep 2.
The incorporation between nano-polyvinyl alcohol (PVA) and nano-chitosan (Cs) to produce sandwich nanohybrid (SNH) for water treatment and improvement the adsorption of sofosbuvir drug (SOF). The photocatalytic activity and formation of reactive oxygen species (ROS) were detected with oxidation of organic dyes such as Rhodamine B (RhB), methylene blue (MB), and methyl orange (MO). The effect of SNH on the release of SOF in blood and inside the cells at pH 7.4 and pH 6.8, respectively were observed by UV-Visible spectroscopy (UV-Vis). The binding constant (K) was reported at 0.0035 min and the loading constant at 0.0024 min, while the release efficiency was 42.6% at pH 7.4 and 74.7% at pH 6.8. The efficiency of photocatalytic activity against organic dyes MO, MB, and RhB are detected at 2.4% and 1%, and 42%, respectively. The cytotoxicity of SNH has been observed with MDA-MB-231 and HepG2 cell line with three concentrations of SNH, where the little concentration has low effect on the HepG2 and high viability, this result was reversed with the high concentration, also the yellow color due to the lysis of the cells. The antioxidant of the SNH was detected by FRAP technique.
将纳米聚乙烯醇 (PVA) 和纳米壳聚糖 (Cs) 结合起来制备三明治纳米杂化材料 (SNH) 以用于水处理，并提高索非布韦药物 (SOF) 的吸附性。通过氧化罗丹明 B (RhB)、亚甲蓝 (MB) 和甲基橙 (MO) 等有机染料来检测光催化活性和活性氧物种 (ROS) 的形成。通过紫外可见光谱 (UV-Vis) 观察 SNH 在 pH 7.4 和 pH 6.8 下分别对血液和细胞内 SOF 释放的影响。报道了结合常数 (K) 为 0.0035 min，负载常数为 0.0024 min，而在 pH 7.4 时的释放效率为 42.6%，在 pH 6.8 时的释放效率为 74.7%。SNH 对 MO、MB 和 RhB 等有机染料的光催化活性效率分别为 2.4%、1%和 42%。用 MDA-MB-231 和 HepG2 细胞系观察了 SNH 的细胞毒性，并用三个浓度的 SNH 处理，其中低浓度对 HepG2 的影响较小，细胞活力较高，而高浓度则相反，细胞裂解导致溶液变黄。通过 FRAP 技术检测了 SNH 的抗氧化性。
