Maitra P K, Lobo Z
Mol Gen Genet. 1977 Nov 4;156(1):55-60. doi: 10.1007/BF00272252.
A mutation pgi1 in the yeast Saccharomyces cerevisiae conferring deficiency of the glycolytic enzyme glucose 6-phosphate isomerase is characterised genetically. The mutation segregates 2+:2- in tetrads from diploids heterozygous for the mutant phenotype. The mutation is semi-dominant and is located on the right arm of chromosome II in the order: tsm134--lys2--pgi1--tyr1 approximately 15 map units from tyr1. The mutation pgi1 defines the structural gene of glucose 6-phosphate isomerase and can be suqpressed intragenically giving revertants that have an unstable enzyme. In one temperature-sensitive revertant no enzyme activity in excess of the mutant level could be detected although fructose 6-phosphate was converted to glucose 6-phosphate in vivo. The suppressor locus in this revertant is dominant and is unlinked to the pgi1 locus.
对酿酒酵母中赋予糖酵解酶葡萄糖 6 -磷酸异构酶缺陷的pgi1突变进行了遗传学特征分析。该突变在来自突变表型杂合二倍体的四分体中以2 +:2 - 分离。该突变是半显性的，位于染色体II右臂上，顺序为：tsm134--lys2--pgi1--tyr1，距tyr1约15个遗传单位。pgi1突变定义了葡萄糖 6 -磷酸异构酶的结构基因，并且可以通过基因内抑制产生具有不稳定酶的回复体。在一个温度敏感型回复体中，尽管在体内6 -磷酸果糖被转化为6 -磷酸葡萄糖，但未检测到超过突变水平的酶活性。该回复体中的抑制基因座是显性的，并且与pgi1基因座不连锁。
