Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran; Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
Res Microbiol. 2021 Jun-Aug;172(4-5):103849. doi: 10.1016/j.resmic.2021.103849. Epub 2021 Jun 2.
Co-infection with other microorganisms can promote the Candida albicans to be invasive. In this study, Escherichia coli and C. albicans were co-isolated from the women with candidiasis symptoms. The in vitro effects of E. coli on C. albicans hypha development, biofilm formation, antibiotic susceptibility, dispersion from the biofilm, expression of Als3, Hwp1, and Tup1 genes, and pathogenesis in Galleria mellonella were investigated. Electron microscopic images revealed that hypha induction was markedly increased in the bacteria-fungi co-culture. Biofilm formation was increased 2.2 fold in the presence of E. coli. The minimum inhibitory concentration of nystatin against Candida was increased from (μg mL) 25 to 50 in the dual biofilm. Candida dissemination was increased up to 2.7 fold from the mixed fungi/bacteria biofilm. The expression of ALS3 and HWP1 genes was increased (5.9 and 2.0 fold, respectively) while the TUP1 gene expression was decreased (0.4 fold) when C. albicans was incubated with E. coli. The simultaneous injection of C. albicans and E. coli to the insect larvae increased Galleria mortality up to 40%. This study demonstrated the effects of E. coli to promote fungi virulence factors, which suggest polymicrobial interaction should be considered during treatment of fungal infections.
其他微生物的共感染可促进白色念珠菌的侵袭性。在这项研究中，从患有念珠菌病症状的女性中共同分离出大肠杆菌和白色念珠菌。研究了大肠杆菌对白色念珠菌菌丝发育、生物膜形成、抗生素敏感性、从生物膜中分散、Als3、Hwp1 和 Tup1 基因表达以及在大蜡螟中的发病机制的体外影响。电子显微镜图像显示，在细菌-真菌共培养物中菌丝诱导明显增加。大肠杆菌的存在使生物膜形成增加了 2.2 倍。在双生物膜中，制霉菌素对念珠菌的最小抑菌浓度从（μg mL）25 增加到 50。从混合真菌/细菌生物膜中，白色念珠菌的传播增加了高达 2.7 倍。当白色念珠菌与大肠杆菌孵育时，ALS3 和 HWP1 基因的表达分别增加了（5.9 和 2.0 倍），而 TUP1 基因的表达减少了（0.4 倍）。同时向昆虫幼虫中注射白色念珠菌和大肠杆菌可使大蜡螟死亡率增加到 40%。本研究证明了大肠杆菌促进真菌毒力因子的作用，这表明在治疗真菌感染时应考虑多微生物相互作用。
