Kennedy C
Mol Gen Genet. 1977 Nov 29;157(2):199-204. doi: 10.1007/BF00267398.
The nif cistrons indentified by complementation analysis in the preceding paper (Dixon et al., 1977) were mapped with respect to hisD and to each other other by P1 cotransduction and three-factor reciprocal crosses. The order obrained was hisD nifB nifA (nifL) nifF nifE nifK nifD nifH. Analysis of hisD2-nif cotransduction data by the Wu equation (Wu, 1966) suggested that the nif genes are divided into two clusters: a his-proximal cluster comprising nifBA(L)F and a his-distal group of nifEKDH.
在前一篇论文(Dixon等人,1977年)中通过互补分析鉴定出的nif顺反子,通过P1共转导以及三因子相互杂交,相对于hisD以及彼此之间进行了定位。得到的顺序是hisD nifB nifA(nifL)nifF nifE nifK nifD nifH。用Wu方程(Wu,1966年)对hisD2-nif共转导数据进行分析表明,nif基因分为两个簇:一个是包含nifBA(L)F的his近端簇,以及一个nifEKDH的his远端组。