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肺炎克雷伯菌中固氮(nif)基因的连锁图谱。

Linkage map of the nitrogen fixation (nif) genes in Klebsiella pneumoniae.

作者信息

Kennedy C

出版信息

Mol Gen Genet. 1977 Nov 29;157(2):199-204. doi: 10.1007/BF00267398.

DOI:10.1007/BF00267398
PMID:340924
Abstract

The nif cistrons indentified by complementation analysis in the preceding paper (Dixon et al., 1977) were mapped with respect to hisD and to each other other by P1 cotransduction and three-factor reciprocal crosses. The order obrained was hisD nifB nifA (nifL) nifF nifE nifK nifD nifH. Analysis of hisD2-nif cotransduction data by the Wu equation (Wu, 1966) suggested that the nif genes are divided into two clusters: a his-proximal cluster comprising nifBA(L)F and a his-distal group of nifEKDH.

摘要

在前一篇论文(Dixon等人,1977年)中通过互补分析鉴定出的nif顺反子,通过P1共转导以及三因子相互杂交,相对于hisD以及彼此之间进行了定位。得到的顺序是hisD nifB nifA(nifL)nifF nifE nifK nifD nifH。用Wu方程(Wu,1966年)对hisD2-nif共转导数据进行分析表明,nif基因分为两个簇:一个是包含nifBA(L)F的his近端簇,以及一个nifEKDH的his远端组。

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引用本文的文献

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EMBO J. 1982;1(2):197-204. doi: 10.1002/j.1460-2075.1982.tb01147.x.
2
The NifL-NifA System: a multidomain transcriptional regulatory complex that integrates environmental signals.NifL-NifA系统:一种整合环境信号的多结构域转录调控复合体。
J Bacteriol. 2004 Feb;186(3):601-10. doi: 10.1128/JB.186.3.601-610.2004.
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Nitrogen control of the nif regulon in Klebsiella pneumoniae: involvement of the ntrA gene and analogies between ntrC and nifA.

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Direct selection for P1-sensitive mutants of enteric bacteria.对肠道细菌的P1敏感突变体进行直接筛选。
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The nitrogen fixation genes.固氮基因。
Nature. 1972 Oct 27;239(5374):495-9. doi: 10.1038/239495a0.
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Biochem J. 1972 Jul;128(3):655-75. doi: 10.1042/bj1280655.
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Regulation of nitrogen fixation in Klebsiella pneumoniae: evidence for a role of glutamine synthetase as a regulator of nitrogenase synthesis.肺炎克雷伯菌中固氮作用的调控:谷氨酰胺合成酶作为固氮酶合成调节剂作用的证据。
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Biochemistry and genetics of Klebsiella pneumoniae mutant strains unable to fix N2.肺炎克雷伯菌无法固氮的突变菌株的生物化学与遗传学
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Use of bacteriophage Mu to isolate deletions in the his-nif region of Klebsiella pneumoniae.利用噬菌体Mu分离肺炎克雷伯菌his-nif区域的缺失突变体。
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