对肠道细菌的P1敏感突变体进行直接筛选。
Direct selection for P1-sensitive mutants of enteric bacteria.
作者信息
Goldberg R B, Bender R A, Streicher S L
出版信息
J Bacteriol. 1974 Jun;118(3):810-4. doi: 10.1128/jb.118.3.810-814.1974.
Abstract
A method has been developed to isolate mutants sensitive to coliphage P1 from bacterial genera normally not sensitive to this phage. P1clr100KM was used. This phage is heat inducible and confers kanamycin resistance when present as a prophage (in lysogens). P1-sensitive mutants of Klebsiella, Enterobacter, Citrobacter, and Erwinia have been found. This technique provides a well-known genetic system for the study of many bacterial genera that previously had either no such system or only a marginally useful means of genetic manipulation. It also extends the range of possible intergeneric hybrids that may be constructed and studied.
摘要
已开发出一种方法，用于从通常对噬菌体P1不敏感的细菌属中分离对大肠杆菌噬菌体P1敏感的突变体。使用了P1clr100KM。这种噬菌体是热诱导型的，当作为原噬菌体（在溶源菌中）存在时赋予卡那霉素抗性。已发现克雷伯氏菌属、肠杆菌属、柠檬酸杆菌属和欧文氏菌属的P1敏感突变体。这项技术为研究许多细菌属提供了一个知名的遗传系统，这些细菌属以前要么没有这样的系统，要么只有一种用处不大的基因操作手段。它还扩展了可能构建和研究的属间杂种的范围。
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