Intravital microscopy, such as 2-photon microscopy, is now a mainstay in immunological research to visually characterize immune cell dynamics during homeostasis and pathogen infections. This approach has been especially beneficial in describing the complex process of host immune responses to parasitic infections , such as Human-parasite co-evolution has endowed parasites with multiple strategies to subvert host immunity in order to establish chronic infections and ensure human-to-human transmission. While much focus has been placed on viral and bacterial infections, intravital microscopy studies during parasitic infections have been comparatively sparse. In this review, we will discuss how microscopy has provided important insights into the generation of innate and adaptive immunity in various organs during parasitic infections, with a primary focus on . We highlight how microscopy-based approaches may be key to providing mechanistic insights into persistence and to devise strategies for better parasite control.