Liang Wenyu, Yan Fengkun, Wang Meng, Li Xiaoxu, Zhang Zheng, Ma Xiaorong, Hu Jinhong, Wang Jun, Wang Lingxia
School of Life Sciences, Ningxia University, Yinchuan 750021, China.
School of Agriculture, Ningxia University, Yinchuan 750021, China.
ACS Omega. 2021 May 17;6(21):13554-13566. doi: 10.1021/acsomega.0c06111. eCollection 2021 Jun 1.
Terrestrial cyanobacteria, originated from aquatic cyanobacteria, exhibit a unique mechanism for drought adaptation during long-term evolution. To elucidate this diverse adaptive mechanism exhibited by terrestrial cyanobacteria from the post-translation modification aspect, we performed a global phosphoproteome analysis on the abundance of phosphoproteins in response to dehydration using , a kind of terrestrial cyanobacteria having strong ecological adaptability to xeric environments. A total of 329 phosphopeptides from 271 phosphoproteins with 1168 phosphorylation sites were identified. Among these, 76 differentially expressed phosphorylated proteins (DEPPs) were identified for each dehydration treatment (30, 75, and 100% water loss), compared to control. The identified DEPPs were functionally categorized to be mainly involved in a two-component signaling pathway, photosynthesis, energy and carbohydrate metabolism, and an antioxidant system. We concluded that protein phosphorylation modifications related to the reactive oxygen species (ROS) signaling pathway might play an important role in coordinating enzyme activity involved in the antioxidant system in to adapt to dehydration stress. This study provides deep insights into the extensive modification of phosphorylation in terrestrial cyanobacteria using a phosphoproteomic approach, which may help to better understand the role of protein phosphorylation in key cellular mechanisms in terrestrial cyanobacteria in response to dehydration.
陆生蓝藻起源于水生蓝藻，在长期进化过程中展现出独特的干旱适应机制。为了从翻译后修饰层面阐明陆生蓝藻所表现出的这种多样的适应机制，我们使用一种对干旱环境具有较强生态适应性的陆生蓝藻—— ，对脱水响应过程中磷蛋白的丰度进行了全磷蛋白质组分析。共鉴定出271个磷蛋白中的329个磷酸肽，具有1168个磷酸化位点。其中，与对照相比，每种脱水处理（水分损失30%、75%和100%）均鉴定出76个差异表达的磷酸化蛋白（DEPPs）。所鉴定的DEPPs在功能上主要归类于双组分信号通路、光合作用、能量和碳水化合物代谢以及抗氧化系统。我们得出结论，与活性氧（ROS）信号通路相关的蛋白质磷酸化修饰可能在协调 中参与抗氧化系统的酶活性以适应脱水胁迫方面发挥重要作用。本研究通过磷蛋白质组学方法深入洞察了陆生蓝藻中磷酸化的广泛修饰，这可能有助于更好地理解蛋白质磷酸化在陆生蓝藻响应脱水的关键细胞机制中的作用。
