Hubei Key Lab of Genetic Regulation & Integrative Biology, School of Life Sciences, Central China Normal University, No. 152 Luoyu Road, Wuhan 430079, PR China.
Hubei Key Lab of Genetic Regulation & Integrative Biology, School of Life Sciences, Central China Normal University, No. 152 Luoyu Road, Wuhan 430079, PR China.
J Proteomics. 2019 Feb 10;192:160-168. doi: 10.1016/j.jprot.2018.08.019. Epub 2018 Sep 4.
To adapt to xeric environments, microorganisms have evolved with the capability of the superior desiccation tolerance and rapid resuscitation after rehydration. Nostoc flagelliforme, a representative terrestrial cyanobacterium that is distributed in west and west-northern parts of China, serves as an ideal model for gaining insight in the physiological recovery mechanism. In this study, LC-MS/MS combined with isobaric chemical labeling technique (iTRAQ) was used to quantify dynamic changes of proteins in N. flagelliforme during the rehydration processes. Approximately 113 proteins were identified to be differentially expressed, with function mainly related to photosynthesis, defense response, biosynthesis, antioxidant system, and energy and carbohydrate metabolism. Among them, protective proteins including high light inducible proteins and antioxidants showed a down regulation trend during the rehydration process, while proteins involved in photosynthesis, biosynthesis and signaling pathways and regulation of gene expression tend to be up-regulated. These results might shed light on molecular mechanism for the N.flagelliforme response to hydration. SIGNIFICANCE: In this work, iTRAQ-based proteome expression profiling provides a holistic proteomic insight for N. flagelliforme in response to rehydration processes. Proteins involved in defense system could help to limit the damage to a repairable level and maintain cellular physiological integrity in the dried state. In addition, results in this work suggest that changes in expression of light-harvesting complexes phycobilisome is closely related to the switch of photosynthesis apparatus, while only a few proteins in PSI and PSII present significant expression change, which may indicate the integrity of PSI and PSII photosynthetic system.
为了适应干旱环境，微生物进化出了卓越的干燥耐受性和再水合后的快速复苏能力。发菜，一种分布在中国西部和西北部的典型陆生蓝细菌，是研究生理恢复机制的理想模型。在这项研究中，LC-MS/MS 结合等压化学标记技术（iTRAQ）被用于定量分析发菜在再水合过程中蛋白质的动态变化。大约有 113 种蛋白质被鉴定为差异表达，其功能主要与光合作用、防御反应、生物合成、抗氧化系统以及能量和碳水化合物代谢有关。其中，包括高光诱导蛋白和抗氧化剂在内的保护性蛋白在再水合过程中呈下调趋势，而参与光合作用、生物合成和信号通路以及基因表达调控的蛋白则呈上调趋势。这些结果可能为发菜对水合作用的响应提供分子机制方面的启示。意义：在这项工作中，基于 iTRAQ 的蛋白质组表达谱分析为发菜对再水合过程的响应提供了全面的蛋白质组学见解。防御系统相关蛋白有助于将损伤限制在可修复水平，并在干燥状态下维持细胞生理完整性。此外，本工作的结果表明，捕光复合物藻胆体表达的变化与光合作用装置的转换密切相关，而 PSI 和 PSII 中只有少数蛋白质的表达发生显著变化，这可能表明 PSI 和 PSII 光合作用系统的完整性。
