Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, China.
Xichang College, Xichang, 615000, China.
Mol Biol Rep. 2021 May;48(5):4341-4350. doi: 10.1007/s11033-021-06449-z. Epub 2021 Jun 7.
Tartary buckwheat is a kind of plant which can be used as medicine as well as edible. Abscisic acid (ABA) signaling plays an important role in the response of plants such as tartary buckwheat to drought and other stress. However, there are not many studies on tartary buckwheat by ABA treatment. In this study, the germination, root length, stoma, and anthocyanin accumulation of tartary buckwheat were all significantly affected by ABA. ABA signaling is important for plants to respond to drought and other stresses, the bZIP gene family is an important member of the ABA signaling pathway. Through the analysis of the origin relationship between tartary buckwheat bZIP family and its related species, 19 bZIP genes in tartary buckwheat were found to be relatively conserved, which laid a foundation for further study of bZIP family. The qRT-PCR results showed that most of the group members were induced by ABA treatment, including 0, 15, 30, 50, 70 µM ABA and 0, 0.5, 2, 4, 8, 16, 24 h ABA treatment. These results suggested that ABA could affect the growth and development of tartary buckwheat, and FtbZIPs might have different functions in the response of tartary buckwheat to drought. This study will be helpful to further analyze the genetic breeding and economic value of tartary buckwheat resistance.
鞑靼荞麦是一种兼具药用和食用价值的植物。脱落酸（ABA）信号在植物（如鞑靼荞麦）对干旱和其他胁迫的响应中起着重要作用。然而，关于 ABA 处理对鞑靼荞麦的研究并不多。在这项研究中，ABA 显著影响了鞑靼荞麦的萌发、根长、气孔和花青素积累。ABA 信号对于植物应对干旱和其他胁迫至关重要，bZIP 基因家族是 ABA 信号通路的重要成员。通过分析鞑靼荞麦 bZIP 家族与其相关物种的起源关系，发现鞑靼荞麦有 19 个 bZIP 基因相对保守，这为进一步研究 bZIP 家族奠定了基础。qRT-PCR 结果表明，大多数成员都被 ABA 处理诱导，包括 0、15、30、50、70μM ABA 和 0、0.5、2、4、8、16、24 h ABA 处理。这些结果表明，ABA 可以影响鞑靼荞麦的生长和发育，FtbZIPs 可能在鞑靼荞麦对干旱的响应中具有不同的功能。这项研究将有助于进一步分析鞑靼荞麦的遗传育种和经济价值。
