Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, School of Food and Biological Engineering, Chengdu University, Chengdu 610106, PR China.
Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, School of Food and Biological Engineering, Chengdu University, Chengdu 610106, PR China.
Int J Biol Macromol. 2021 Feb 28;171:435-447. doi: 10.1016/j.ijbiomac.2021.01.016. Epub 2021 Jan 9.
Tartary buckwheat (Fagopyrum tataricum Gaertn.) is an economical crop with excellent edible, nutritional, and medicinal values. However, the production of Tartary buckwheat is very low and it is urgent to breed high-yield varieties for satisfying the increasing market demand. Heterotrimeric G-protein signaling involves in the regulation of agronomical traits and fruit development in several plant species. In this study, fifteen genes involved in G-protein signaling were characterized in Tartary buckwheat and their potential roles in fruit development were revealed by expression analysis. The exon-intron organization and conserved motif of Tartary buckwheat G-protein signaling genes were similar to those in other dicot plants. All these genes were ubiquitously and differently expressed in five tissues. The expression patterns of Tartary buckwheat G-protein signaling genes in fruit suggested they may play important roles in the fruit at early development stage, which was supported by meta-analysis of G-protein signaling genes' expression in the fruits from different species. Furthermore, we found the expression of G-protein signaling genes in fruit showed high correlation with 178 transcription factors, which indicated a transcriptional regulatory loop moderating G-protein signaling genes' expression during fruit development. This paper provides new insights into the physiological functions of G-protein signaling in fruit.
鞑靼荞麦（Fagopyrum tataricum Gaertn.）是一种经济作物，具有极好的食用、营养和药用价值。然而，鞑靼荞麦的产量非常低，为了满足不断增长的市场需求，迫切需要培育高产品种。异三聚体 G 蛋白信号转导参与几种植物的农艺性状和果实发育的调控。本研究在鞑靼荞麦中鉴定了 15 个参与 G 蛋白信号转导的基因，并通过表达分析揭示了它们在果实发育中的潜在作用。鞑靼荞麦 G 蛋白信号转导基因的exon-intron 组织和保守基序与其他双子叶植物相似。所有这些基因在五个组织中均有广泛而不同的表达。鞑靼荞麦 G 蛋白信号转导基因在果实中的表达模式表明，它们可能在果实早期发育阶段发挥重要作用，这得到了来自不同物种的果实中 G 蛋白信号转导基因表达的荟萃分析的支持。此外，我们发现果实中 G 蛋白信号转导基因的表达与 178 个转录因子高度相关，这表明在果实发育过程中，转录因子调节 G 蛋白信号转导基因的表达形成一个转录调控回路。本文为 G 蛋白信号在果实中的生理功能提供了新的见解。
