• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

采用多种新的神经生物学方法研究小鼠环境神经毒理学。

Employing Multiple New Neurobiological Methods to Investigate Environmental Neurotoxicology in Mice.

机构信息

The Key Laboratory of Environment and Health, School of Public Health, Fujian Medical University, Fuzhou, China.

Department of Preventive Medicine, School of Public Health, Fujian Medical University, Fuzhou, China.

出版信息

Methods Mol Biol. 2021;2326:105-122. doi: 10.1007/978-1-0716-1514-0_8.

DOI:10.1007/978-1-0716-1514-0_8
PMID:34097264
Abstract

The emergence of advanced and powerful neuroscientific technologies has greatly pushed forward the development of neurobiology in the last decade. Although neurotoxicology is an interdisciplinary subject sharing a mass of technologies with neurobiology, the implementation of these advanced technologies in neurotoxicology is merely seen. Here we describe the detailed methods and materials of some emerging neuroscientific technologies, including optogenetics, fiber photometry, in vivo two-photon Imaging, in vivo calcium imaging, and in vivo electrophysiological recording, hoping that the integration of technologies from neurotoxicology and neuroscience can lend weight to the development of neurotoxicology.

摘要

在过去的十年中,先进而强大的神经科学技术的出现极大地推动了神经生物学的发展。尽管神经毒理学是一个与神经生物学共享大量技术的跨学科领域,但这些先进技术在神经毒理学中的应用却鲜为人知。在这里,我们描述了一些新兴神经科学技术的详细方法和材料,包括光遗传学、光纤光度法、在体双光子成像、在体钙成像和在体电生理记录,希望神经毒理学和神经科学技术的融合能够为神经毒理学的发展提供助力。

相似文献

1
Employing Multiple New Neurobiological Methods to Investigate Environmental Neurotoxicology in Mice.采用多种新的神经生物学方法研究小鼠环境神经毒理学。
Methods Mol Biol. 2021;2326:105-122. doi: 10.1007/978-1-0716-1514-0_8.
2
Long-term Fiber Photometry for Neuroscience Studies.长期光纤光度测定在神经科学研究中的应用。
Neurosci Bull. 2019 Jun;35(3):425-433. doi: 10.1007/s12264-019-00379-4. Epub 2019 May 6.
3
Open source silicon microprobes for high throughput neural recording.开源硅微探针,用于高通量神经记录。
J Neural Eng. 2020 Jan 24;17(1):016036. doi: 10.1088/1741-2552/ab581a.
4
Fiber photometry in striatum reflects primarily nonsomatic changes in calcium.纹状体中的光纤光度测定主要反映钙的非体细胞变化。
Nat Neurosci. 2022 Sep;25(9):1124-1128. doi: 10.1038/s41593-022-01152-z. Epub 2022 Aug 30.
5
Protocol for fiber photometry recording from deep brain regions in head-fixed mice.头部固定小鼠深部脑区光纤光度记录的方案。
STAR Protoc. 2024 Jun 21;5(2):103131. doi: 10.1016/j.xpro.2024.103131. Epub 2024 Jun 13.
6
The Application of Omics Technologies in the Research of Neurotoxicology.组学技术在神经毒理学研究中的应用。
Methods Mol Biol. 2021;2326:143-154. doi: 10.1007/978-1-0716-1514-0_10.
7
Neurophysiological Assessment of Huntington's Disease Model Mice.亨廷顿舞蹈症模型小鼠的神经生理学评估
Methods Mol Biol. 2018;1780:163-177. doi: 10.1007/978-1-4939-7825-0_9.
8
Stretchable Transparent Electrode Arrays for Simultaneous Electrical and Optical Interrogation of Neural Circuits in Vivo.可拉伸透明电极阵列,用于在体同时进行神经回路的电和光检测。
Nano Lett. 2018 May 9;18(5):2903-2911. doi: 10.1021/acs.nanolett.8b00087. Epub 2018 Apr 9.
9
High-density multi-fiber photometry for studying large-scale brain circuit dynamics.用于研究大脑大规模电路动力学的高密度多光纤光度测定法。
Nat Methods. 2019 Jun;16(6):553-560. doi: 10.1038/s41592-019-0400-4. Epub 2019 May 13.
10
pMAT: An open-source software suite for the analysis of fiber photometry data.pMAT:一个用于光纤光度数据分析的开源软件套件。
Pharmacol Biochem Behav. 2021 Feb;201:173093. doi: 10.1016/j.pbb.2020.173093. Epub 2020 Dec 29.

本文引用的文献

1
Basal forebrain GABAergic neurons promote arousal and predatory hunting.基底前脑γ-氨基丁酸能神经元促进觉醒和捕食行为。
Neuropharmacology. 2020 Dec 1;180:108299. doi: 10.1016/j.neuropharm.2020.108299. Epub 2020 Sep 9.
2
Miniscope GRIN Lens System for Calcium Imaging of Neuronal Activity from Deep Brain Structures in Behaving Animals.用于行为动物深部脑结构神经元活动钙成像的微型显微镜梯度折射率透镜系统。
Curr Protoc Neurosci. 2019 Jan;86(1):e56. doi: 10.1002/cpns.56. Epub 2018 Oct 13.
3
Environmental toxicology: Sensitive periods of development and neurodevelopmental disorders.
环境毒理学:发育敏感期与神经发育障碍
Neurotoxicology. 2017 Jan;58:23-41. doi: 10.1016/j.neuro.2016.10.017. Epub 2016 Nov 4.
4
Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging.用于体内双光子成像的病毒注射和颅骨窗口植入
Methods Mol Biol. 2016;1474:171-85. doi: 10.1007/978-1-4939-6352-2_10.
5
Health Consequences of Environmental Exposures: Changing Global Patterns of Exposure and Disease.环境暴露对健康的影响:暴露和疾病的全球模式变化。
Ann Glob Health. 2016 Jan-Feb;82(1):10-9. doi: 10.1016/j.aogh.2016.01.005.
6
Calcium Imaging of Basal Forebrain Activity during Innate and Learned Behaviors.先天与习得行为期间基底前脑活动的钙成像
Front Neural Circuits. 2016 May 9;10:36. doi: 10.3389/fncir.2016.00036. eCollection 2016.
7
Basal Forebrain Cholinergic Neurons Primarily Contribute to Inhibition of Electroencephalogram Delta Activity, Rather Than Inducing Behavioral Wakefulness in Mice.基底前脑胆碱能神经元主要对小鼠脑电图δ活动的抑制起作用,而非诱导行为觉醒。
Neuropsychopharmacology. 2016 Jul;41(8):2133-46. doi: 10.1038/npp.2016.13. Epub 2016 Jan 22.
8
Environmental chemical exposures and human epigenetics.环境化学暴露与人类表观遗传学。
Int J Epidemiol. 2012 Feb;41(1):79-105. doi: 10.1093/ije/dyr154. Epub 2011 Dec 13.
9
Developmental neurotoxicity of industrial chemicals.工业化学品的发育神经毒性
Lancet. 2006 Dec 16;368(9553):2167-78. doi: 10.1016/S0140-6736(06)69665-7.
10
In vivo two-photon microscopy study of short-term effects of microbeam irradiation on normal mouse brain microvasculature.微束辐照对正常小鼠脑微血管短期影响的体内双光子显微镜研究
Int J Radiat Oncol Biol Phys. 2006 Apr 1;64(5):1519-27. doi: 10.1016/j.ijrobp.2005.11.047.