The Key Laboratory of Environment and Health, School of Public Health, Fujian Medical University, Fuzhou, China.
Department of Preventive Medicine, School of Public Health, Fujian Medical University, Fuzhou, China.
Methods Mol Biol. 2021;2326:105-122. doi: 10.1007/978-1-0716-1514-0_8.
The emergence of advanced and powerful neuroscientific technologies has greatly pushed forward the development of neurobiology in the last decade. Although neurotoxicology is an interdisciplinary subject sharing a mass of technologies with neurobiology, the implementation of these advanced technologies in neurotoxicology is merely seen. Here we describe the detailed methods and materials of some emerging neuroscientific technologies, including optogenetics, fiber photometry, in vivo two-photon Imaging, in vivo calcium imaging, and in vivo electrophysiological recording, hoping that the integration of technologies from neurotoxicology and neuroscience can lend weight to the development of neurotoxicology.
在过去的十年中,先进而强大的神经科学技术的出现极大地推动了神经生物学的发展。尽管神经毒理学是一个与神经生物学共享大量技术的跨学科领域,但这些先进技术在神经毒理学中的应用却鲜为人知。在这里,我们描述了一些新兴神经科学技术的详细方法和材料,包括光遗传学、光纤光度法、在体双光子成像、在体钙成像和在体电生理记录,希望神经毒理学和神经科学技术的融合能够为神经毒理学的发展提供助力。