Brain Research Institute, University of Zurich, Zurich, Switzerland.
Neuroscience Center Zurich, Zurich, Switzerland.
Nat Methods. 2019 Jun;16(6):553-560. doi: 10.1038/s41592-019-0400-4. Epub 2019 May 13.
Animal behavior originates from neuronal activity distributed across brain-wide networks. However, techniques available to assess large-scale neural dynamics in behaving animals remain limited. Here we present compact, chronically implantable, high-density arrays of optical fibers that enable multi-fiber photometry and optogenetic perturbations across many regions in the mammalian brain. In mice engaged in a texture discrimination task, we achieved simultaneous photometric calcium recordings from networks of 12-48 brain regions, including striatal, thalamic, hippocampal and cortical areas. Furthermore, we optically perturbed subsets of regions in VGAT-ChR2 mice by targeting specific fiber channels with a spatial light modulator. Perturbation of ventral thalamic nuclei caused distributed network modulation and behavioral deficits. Finally, we demonstrate multi-fiber photometry in freely moving animals, including simultaneous recordings from two mice during social interaction. High-density multi-fiber arrays are versatile tools for the investigation of large-scale brain dynamics during behavior.
动物行为源于分布在整个大脑网络中的神经元活动。然而，用于评估行为动物中大规模神经动力学的可用技术仍然有限。在这里，我们提出了紧凑型、慢性可植入的高密度光纤阵列，该阵列可实现多光纤光度测量和光遗传学刺激在哺乳动物大脑的许多区域。在参与纹理辨别任务的小鼠中，我们实现了来自 12-48 个脑区网络的同时钙光度记录，包括纹状体、丘脑、海马和皮质区。此外，我们通过使用空间光调制器靶向特定光纤通道，对 VGAT-ChR2 小鼠的区域亚群进行光遗传扰动。腹侧丘脑核的扰动导致分布式网络调制和行为缺陷。最后，我们在自由活动的动物中展示了多光纤光度测量，包括在社交互动期间对两只小鼠的同时记录。高密度多光纤阵列是研究行为过程中大规模大脑动力学的多功能工具。
