College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea.
Department of Animal Science and Technology, Konkuk University, Seoul 05029, Republic of Korea.
J Microbiol Biotechnol. 2021 Jul 28;31(7):956-966. doi: 10.4014/jmb.2103.03024.
To prevent the outbreak of infectious diseases that inflict huge economic and social losses, domestic livestock farms and related facilities have introduced automatic and semiautomatic disinfectant solution-spraying systems for vehicles. However, the facility standards and specifications vary by manufacturer, and no scientific performance evaluation has been conducted. The puropose of this study is to develop physical and biological evaluation methods. Physical and biological appraisals were conducted using two types of disinfection facilities (tunnel- and U-type) and two types of vehicles (passenger car, truck). Water-sensitive paper was used to evaluate the physical performance values for the disinfection facilities. In addition, to assess their biological performance, carriers containing low-pathogenic avian influenza virus were attached to vehicles, and the viral reduction was measured after the vehicles moved through the facility. The tunnel-type had rates of coverage in the range of 70-90% for the passenger car and 60-90% for the truck. At least 4-log virus reduction after spraying for 1-5 min was shown for both vehicles. For the U-type facility evaluation, the coverage rates were in the range of 60-90% for the passenger car and at least 90% for the truck. More than 4-log viral reduction was estimated within a spraying time of 5 min. To reduce viruses on the surface of vehicles by at least 4 log within a short period, the disinfectant solution should cover at least 71% of the pathogens. In conclusion, we were able to assess the physical and biological performance criteria for disinfection facilities aboard transportation vehicles.
为防止造成巨大经济和社会损失的传染病爆发，国内家畜养殖场及相关设施已为车辆引入了自动和半自动消毒剂喷雾系统。然而，各制造商的设施标准和规格有所不同，且尚未进行科学的性能评估。本研究的目的是开发物理和生物评估方法。使用两种类型的消毒设施（隧道式和 U 型）和两种类型的车辆（乘用车、卡车）对物理和生物性能进行评估。采用水敏纸评估了消毒设施的物理性能值。此外，为了评估其生物性能，将含有低致病性禽流感病毒的载体附着在车辆上，并在车辆通过设施后测量病毒减少量。对于隧道式设施，乘用车的覆盖率范围为 70-90%，卡车的覆盖率范围为 60-90%。两种车辆的喷雾 1-5 分钟后，病毒减少率至少为 4 对数。对于 U 型设施的评估，乘用车的覆盖率范围为 60-90%，卡车的覆盖率至少为 90%。估计在 5 分钟的喷雾时间内，病毒减少量超过 4 对数。为了在短时间内将车辆表面的病毒减少至少 4 对数，消毒剂溶液应至少覆盖 71%的病原体。总之，我们能够评估运输车辆上的消毒设施的物理和生物性能标准。
