Jefo Nutrition Inc., Saint-Hyacinthe, QC, Canada.
Department of Animal and Food Sciences, University of Delaware, DE, USA.
Poult Sci. 2021 Jul;100(7):101176. doi: 10.1016/j.psj.2021.101176. Epub 2021 Apr 20.
We evaluated the supplementation of a protected complex of biofactors and antioxidants [P(BF+AOx)] on growth performance, antioxidant activity, expression of immune-related genes, and immunometabolic phenotype of broilers submitted to early life stressors. The treatments were a nutritionally complete basal diet supplemented or not with P(BF+AOx) (Jefo Nutrition Inc., Saint-Hyacinthe, QC, Canada) from 1 to 14 d of age. 720 one-day old male Ross 308 chickens were placed into pens of 30 birds (12 replicates/treatment). Birds were double-vaccinated against infectious bronchitis (IB; MILDVAC-Ma5) at the hatchery and submitted, on d 3, to an acute reduction on environmental temperature (from 32° C to 20°C) for 48 h. Feed intake (FI), body weight gain (BWG), and feed conversion ratio (FCR) were calculated weekly. On d 7 and 15, samples were collected for expression of immune-related genes and kinome array analysis, and serum to evaluate the antioxidant status. Data were analyzed by ANOVA using SAS (SAS 9.4). From d 1 to 21 and d 1 to 28, the dietary supplementation of P(BF+AOx) significantly increased BWG (P < 0.05) by 3.6 and 3.8%, respectively, and improved FCR (P < 0.05) by 1.2 and 1.8%, respectively. From d 1 to 35, dietary supplementation enhanced BWG (P = 0.03) by 4%. Serum glutathione reductase activity on d 15 was higher in birds fed diets supplemented with P(BF+AOx) compared to the control diet-fed birds (P = 0.04). Dietary supplementation reduced the expression of IL-1β (P = 0.03) in the lungs on d 7. On d 15, dietary supplementation increased the expression of IL-6 (P = 0.02) and IL-10 (P = 0.03) in the liver. It was observed that, via decreased phosphorylation, catalase was activated in the jejunum and liver, and the phosphorylation of immunoregulatory or proinflammatory proteins was decreased. Other important cellular signaling pathways were also changed in the liver and jejunum due to the supplementation. The supplementation of P(BF+AOx) improves growth performance by promoting a general anti-inflammatory and antioxidant response in chickens undergoing early life stress.
我们评估了一种受保护的生物因子和抗氧化剂复合物 [P（BF+AOx）] 对生长性能、抗氧化活性、免疫相关基因表达和肉鸡免疫代谢表型的影响，这些肉鸡在早期生活中受到应激源的影响。处理方法是在 1 至 14 日龄时，用营养完整的基础日粮补充或不补充 P（BF+AOx）（Jefo Nutrition Inc.，Saint-Hyacinthe，QC，加拿大）。720 只 1 日龄雄性罗斯 308 肉鸡被放置在 30 只鸡的围栏中（12 个重复/处理）。鸡在孵化场接受传染性支气管炎（IB；MILDVAC-Ma5）的双重疫苗接种，并在第 3 天，将环境温度从 32°C 急性降至 20°C，持续 48 小时。每周计算采食量（FI）、体重增加（BWG）和饲料转化率（FCR）。在第 7 天和第 15 天，收集免疫相关基因的表达和激酶组阵列分析的样本，并收集血清以评估抗氧化状态。使用 SAS（SAS 9.4）进行方差分析。从第 1 天到第 21 天和第 1 天到第 28 天，日粮中添加 P（BF+AOx）分别显著提高了 BWG（P < 0.05）3.6%和 3.8%，并分别提高了 FCR（P < 0.05）1.2%和 1.8%。从第 1 天到第 35 天，日粮添加提高了 BWG（P=0.03）4%。第 15 天，与对照组相比，用添加 P（BF+AOx）的日粮喂养的鸡的血清谷胱甘肽还原酶活性更高（P=0.04）。日粮添加降低了第 7 天肺部 IL-1β的表达（P=0.03）。第 15 天，日粮添加增加了肝脏中 IL-6（P=0.02）和 IL-10（P=0.03）的表达。观察到，在空肠和肝脏中，过氧化氢酶通过磷酸化减少而被激活，免疫调节或促炎蛋白的磷酸化减少。由于补充，其他重要的细胞信号通路也在肝脏和空肠中发生改变。添加 P（BF+AOx）通过促进肉鸡早期生活应激时的一般抗炎和抗氧化反应，提高了生长性能。
