Nairn C J, Winesett L, Ferl R J
Department of Botany, University of Florida, Gainesville 32611.
Gene. 1988 May 30;65(2):247-57. doi: 10.1016/0378-1119(88)90461-1.
An 1830-bp genomic DNA segment containing an Arabidopsis thaliana actin gene, AAc1, has been cloned and sequenced. The AAc1 gene is present as a single-copy gene, but at least two other actin-like genes have been detected. Comparison of the nucleotide sequence of AAc1 with other cloned plant actin genes reveals four exons separated by three introns conservatively located in all plant actin genes. The deduced amino acid sequence has also been compared with actin protein sequences from other plants.
一段包含拟南芥肌动蛋白基因AAc1的1830个碱基对的基因组DNA片段已被克隆并测序。AAc1基因作为单拷贝基因存在,但已检测到至少另外两个肌动蛋白样基因。将AAc1的核苷酸序列与其他已克隆的植物肌动蛋白基因进行比较,发现有四个外显子被三个内含子隔开,这些内含子在所有植物肌动蛋白基因中位置保守。推导的氨基酸序列也已与其他植物的肌动蛋白蛋白序列进行了比较。