Kemmerer E C, Lei M, Wu R
Field of Botany, Cornell University, Ithaca, NY 14853.
J Mol Evol. 1991 Mar;32(3):227-37. doi: 10.1007/BF02342745.
We have isolated a cytochrome c gene from Arabidopsis thaliana (cv. Columbia), which is the first cytochrome c gene to be cloned from a higher plant. Genomic DNA blot analysis indicates that there is only one copy of cytochrome c in Arabidopsis. The gene consists of three exons separated by two introns. Gene features such as regulatory regions, codon usage, and conserved splicing-specific sequences are all present and typical of dicotyledonous plant nuclear genes. We have constructed phenograms and cladograms for cytochrome c amino acid sequences and histone H3, alcohol dehydrogenase, and actin DNA sequences. For both cytochrome c and histone H3, Arabidopsis clusters poorly with other higher plants. Instead, it clusters with Neurospora and/or the yeasts. We suggest that perhaps this observation should be considered when using Arabidopsis as a model system for higher plants.
我们从拟南芥（哥伦比亚生态型）中分离出一个细胞色素c基因，这是从高等植物中克隆出的首个细胞色素c基因。基因组DNA印迹分析表明拟南芥中细胞色素c只有一个拷贝。该基因由三个外显子和两个内含子隔开。基因特征，如调控区、密码子使用情况以及保守的剪接特异性序列均存在，且是双子叶植物核基因的典型特征。我们构建了细胞色素c氨基酸序列以及组蛋白H3、乙醇脱氢酶和肌动蛋白DNA序列的系统发育树和分支图。对于细胞色素c和组蛋白H3而言，拟南芥与其他高等植物的聚类效果不佳。相反，它与粗糙脉孢菌和/或酵母聚类在一起。我们建议在将拟南芥用作高等植物的模型系统时，或许应考虑这一观察结果。
