Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Langmuir. 2021 Jun 22;37(24):7548-7555. doi: 10.1021/acs.langmuir.1c01044. Epub 2021 Jun 10.
Understanding the interaction between living cells and a tantalum pentoxide (TaO) gate electrode is important for controlling cell adhesion and functions when developing a cultured-cell-gate field-effect transistor biosensor. In this study, we evaluate the cell adhesion characteristics of the TaO membrane without or with a polydopamine (pDA) coating for chondrocytes, which is expected as a treatment for improving biocompatibility. As a result, the native and pDA-modified TaO membranes are shown to have the appropriate surface tension (35-40 dyn/cm) for the adhesion of chondrocytes owing to the contribution of surface tension to not only the nonspecific adsorption of serum proteins as the scaffold of chondrocytes but also the maintenance of the conformation of serum proteins. In particular, the serum proteins adhere more efficiently to the native TaO membrane than to the pDA-modified ones owing to the relatively smaller surface tension of the native TaO membrane; as a result, the proliferation and production of extracellular matrix (ECM) proteins such as collagen and proteoglycans by chondrocytes are clearly enhanced on the native TaO membrane. Thus, the native TaO membrane shows superior performance for the chondrocyte culture on it compared with the pDA-modified ones.
了解活细胞与五氧化二钽（TaO）栅极之间的相互作用对于开发培养细胞栅场效应晶体管生物传感器时控制细胞黏附和功能非常重要。在这项研究中，我们评估了 TaO 膜在没有或有聚多巴胺（pDA）涂层的情况下对于软骨细胞的黏附特性，这有望作为改善生物相容性的一种处理方法。结果表明，由于表面张力不仅有助于作为软骨细胞支架的血清蛋白的非特异性吸附，还有助于维持血清蛋白的构象，因此天然和 pDA 修饰的 TaO 膜具有适合软骨细胞黏附的适当表面张力（35-40 达因/厘米）。特别是由于天然 TaO 膜的表面张力相对较小，因此血清蛋白更有效地黏附在天然 TaO 膜上，而不是 pDA 修饰的 TaO 膜上；结果，软骨细胞的增殖和细胞外基质（ECM）蛋白如胶原蛋白和糖胺聚糖的产生明显增强。因此，与 pDA 修饰的 TaO 膜相比，天然 TaO 膜在其上培养软骨细胞具有更好的性能。
